SELEX for tubulin affords specific T-rich DNA aptamers

被引：20

作者：

Fukusaki, E

Hasunuma, T

Kajiyama, S

Okazawa, A

Itoh, TJ

Kobayashi, A

机构：

[1] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan

[2] Nagoya Univ, Div Biol Sci, Grad Sch Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2001年 / 11卷 / 22期

关键词：

D O I：

10.1016/S0960-894X(01)00584-4

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We succeeded in acquiring two DNA aptamers that selectively recognize tubulin by the SELEX method. A pool of single-stranded oligo-DNAs including a random region of 59 nucleotides was screened by SELEX for tubulin purified from calf-brain as a target. After 20 repetitions of selection round, the library converged on specific T-rich sequences. The binding activity of T-rich clones was analyzed by the SPR sensor to determine their dissociation constants to be in the order of 10 muM. (C) 2000 Elsevier Science Ltd. All rights reserved. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2927 / 2930

页数：4

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