Extended resolution fluorescence microscopy

Fluorescence microscopy is an essential tool of modern biology, but, like all forms of optical imaging, it is subject to physical limits on its resolving power. In recent years, several exciting techniques have been introduced to exceed these limits, including standing wave microscopy, 4Pi confocal microscopy, 1(5)M and structured illumination microscopy. Several such techniques have been definitively demonstrated for the first time during the past year.