Small Ubiquitin-like Modifier Protein SUMO Enables Plants to Control Growth Independently of the Phytohormone Gibberellin

被引:126
作者
Conti, Lucio [1 ,2 ]
Nelis, Stuart [1 ]
Zhang, Cunjin [1 ]
Woodcock, Ailidh [1 ,6 ]
Swarup, Ranjan [4 ]
Galbiati, Massimo [2 ,3 ]
Tonelli, Chiara [2 ]
Napier, Richard [6 ]
Hedden, Peter [5 ]
Bennett, Malcolm [4 ]
Sadanandom, Ari [1 ]
机构
[1] Univ Durham, Sch Biol & Biomed Sci, Durham DH1 3LE, England
[2] Univ Milan, Dept Life Sci, I-20133 Milan, Italy
[3] Fdn Filarete, I-20139 Milan, Italy
[4] Univ Nottingham, Sch Biosci, Plant Sci Div, Loughborough LE12 5RD, Leics, England
[5] Rothamsted Res, Harpenden AL5 2JQ, Herts, England
[6] Univ Warwick, Sch Life Sci, Coventry CV4 7ES, W Midlands, England
基金
英国生物技术与生命科学研究理事会;
关键词
ARABIDOPSIS-THALIANA; SIGNALING PATHWAY; DELLA PROTEINS; SEED-GERMINATION; RECEPTOR; RESPONSES; REPRESSOR; ENCODES; LIGASE; GID1;
D O I
10.1016/j.devcel.2013.12.004
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Plants survive adverse conditions by modulating their growth in response to a changing environment. Gibberellins (GAs) play a key role in these adaptive responses by stimulating the degradation of growth-repressing DELLA proteins. GA binding to its receptor GID1 enables association of GID1 with DELLAs. This leads to the ubiquitin-mediated proteasomal degradation of DELLAs and consequently growth promotion. We report that DELLA-dependent growth control can be regulated independently of GA. We demonstrate that when a proportion of DELLAs is conjugated to the Small Ubiquitin-like Modifier (SUMO) protein, the extent of conjugation increases during stress. We identify a SUMO-interacting motif in GID1 and demonstrate that SUMO-conjugated DELLA binds to this motif in a GA-independent manner. The consequent sequestration of GID1 by SUMO-conjugated DELLAs leads to an accumulation of non-SUMOylated DELLAs, resulting in beneficial growth restraint during stress. We conclude that plants have developed a GA-independent mechanism to control growth.
引用
收藏
页码:102 / 110
页数:9
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