A charge detector for time-of-flight mass analysis of high mass ions produced by matrix-assisted laser desorption/ionization (MALDI)

被引:32
作者
Bahr, U [1 ]
Rohling, U [1 ]
Lautz, C [1 ]
Strupat, K [1 ]
Schurenberg, M [1 ]
Hillenkamp, F [1 ]
机构
[1] UNIV MUNSTER, INST MED PHYS & BIOPHYS, W-4400 MUNSTER, GERMANY
关键词
charge detector; Faraday cup; ion detector; MALDI; mass resolution; secondary ions; sensitivity; time of flight;
D O I
10.1016/0168-1176(95)04351-9
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
A novel detector for ion detection in MALDI time-of-flight instruments is described. It comprises a hat 18 mm diameter metal electrode as a Faraday charge collector followed by a fast, low noise, charge-sensitive FET impedance converter/amplifier The resulting staircase signals are differentiated by a bandpass filter which can be optimized with respect to rise/fall time and electronic noise. A net charge, equivalent to 1.8 x 10(4) elementary charges, is required for a signal-to-noise ratio of 2. In the low mass range the detector requires a threshold laser intensity for the detection of MALDI analyte ions of about a factor of 1.5 above that needed if a standard secondary electron detector is used. Above an ion mass of approximately 30 kDa, its sensitivity is equal to that of the other detectors. With a rise time of 25 ns, the mass resolution for MALDI ion detection in the mid and high mass range is not limited by the detector. The influence of secondary ion generation at the collector electrode is also reported.
引用
收藏
页码:9 / 21
页数:13
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