Centromeres reposition to the nuclear periphery during L6E9 myogenesis in vitro

被引：54

作者：

Chaly, N

Munro, SB

机构：

[1] Department of Biology, Carleton University, Ottawa, Ont.

[2] Department of Biology, Carleton University, Ottawa, Ont. K1S 5B6

来源：

EXPERIMENTAL CELL RESEARCH | 1996年 / 223卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1006/excr.1996.0082

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To test the hypothesis that genome architecture in interphase is related to nuclear function, we have compared the disposition of centromeres in nuclei of undifferentiated rat L6E9 myoblasts with that in nuclei of LEES myotubes differentiated in vitro. Immunofluorescence labeling showed that centromeres repositioned to the nuclear periphery during differentiation, and condensed chromatin was more prominent at the myotube nuclear envelope by electron microscopy. These data indicate that, in parallel with considerable changes in gene expression, the spatial order of the genome undergoes substantial rearrangement during myogenesis. (C) 1996 Academic Press, Inc.

引用

页码：274 / 278

页数：5

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[21] REPRODUCIBLE COMPARTMENTALIZATION OF INDIVIDUAL CHROMOSOME DOMAINS IN HUMAN CNS CELLS REVEALED BY INSITU HYBRIDIZATION AND 3-DIMENSIONAL RECONSTRUCTION [J].