All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing

被引:43
作者
Esposito, A
Oggier, T
Gerritsen, HC
Lustenberger, F
Wouters, FS
机构
[1] European Neurosci Inst Gottingen, Cell Biophys Grp, D-37073 Gottingen, Germany
[2] Univ Utrecht, Debye Inst, NL-3508 TA Utrecht, Netherlands
[3] Ctr Suisse Elect & Microtech SA, Image Sensing Grp, Photon Div, CH-8048 Zurich, Switzerland
来源
OPTICS EXPRESS | 2005年 / 13卷 / 24期
关键词
D O I
10.1364/OPEX.13.009812
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique that is increasingly being used in the life sciences during the past decades. However, a broader application of FLIM requires more cost-effective and user-friendly solutions. We demonstrate the use of a simple CCD/CMOS lock-in imager for fluorescence lifetime detection. The SwissRanger SR-2 time-of-flight detector, originally developed for 3D vision, embeds all the functionalities required for FLIM in a compact system. The further development of this technology and its combination with light-emitting-and laser diodes could drive a wider spreading of the use of FLIM including high-throughput applications. (c) 2005 Optical Society of America
引用
收藏
页码:9812 / 9821
页数:10
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