Minor constituents from Bupleurum fruticosum roots

A new triterpenoid saponin, 23-acetoxy,16 alpha-hydroxy-13,28-epoxyolean-11-en-3 beta-yl-[beta-D-glucopyranosyl-(1 --> 2)]-[beta-D-glucopyranosil(1 --> 3)]-beta-D-fucopyranoside, has been isolated from the roots of Bupleurum fruticosum, along with eight coumarins, scopoletin, scoparone, prenyletin, capensin, fraxetin, aesculetin,7-(3 -methyl-2-butenyloxy-6 -methoxycoumarin, 7-(2-hydroxy-3-methyl-3-butenyloxy)-6-methoxycoumarin and 5,7-dihydroxy-6-methoxy-8-(3-methyl-2-butenyl)coumarin. The last compound has not been previously encountered in nature. The structures were determined by analysis of NMR spectral data including two-dimensional techniques.