Reactions of isolated mono-molecular protein films

被引:14
作者
Perriman, Adam W. [1 ,2 ]
McGillivray, Duncan J. [1 ,3 ]
White, John W. [1 ]
机构
[1] Australian Natl Univ, Res Sch Chem, Canberra, ACT 0200, Australia
[2] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England
[3] Univ Auckland, Dept Chem, Auckland 1142, New Zealand
基金
澳大利亚研究理事会;
关键词
D O I
10.1039/b804711a
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Wash-out of the forming solution from mono-molecular casein films at the air-water interface is demonstrated and allows the perfusion of the subphase by inorganic and protein solutions. In this way, using X-ray reflectometry, we have observed the effects of calcium binding to b-casein films and the inter-protein association of b-casein and k-casein in an order-specific manner. The method is of general utility for monolayer reactions and here the relevance to self-assembly of the casein micelle is discussed.
引用
收藏
页码:2192 / 2198
页数:7
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