Purification and characterization of microbial gellan lyase

被引：28

作者：

Hashimoto, W

Inose, T

Nakajima, H

Sato, N

Kimura, S

Murata, K

机构：

[1] KYOTO UNIV,FOOD SCI RES INST,UJI,KYOTO 611,JAPAN

[2] MARUHA CO,CENT RES INST,TSUKUBA,IBARAKI 30042,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 04期

关键词：

D O I：

10.1128/AEM.62.4.1475-1477.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Gellan lyase was purified from the culture fluid of soil samples incubated in a medium containing gellan as a sole carbon source. The enzyme was a monomer with a molecular mass of 140 kDa and was most active at pH 7.5 and 45 degrees C. The enzyme was highly specific to gellan and lowered the viscosity of the polymer.

引用

页码：1475 / 1477

页数：3

共 11 条

[1]

Allen F, 1990, US Patent, Patent No. [4 963 668, 4963668]

[2] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].