Evaluation of IgA-mediated humoral immune response against the mycobacterial antigen P-90 in diagnosis of pulmonary tuberculosis

被引:31
作者
Alifano, M
DePascalis, R
Sofia, M
Faraone, S
DelPezzo, M
Covelli, I
机构
[1] UNIV NAPLES FEDERICO II,INST RESP DIS,NAPLES,ITALY
[2] UNIV NAPLES FEDERICO II,DEPT CELLULAR & MOL BIOL & PATHOL,NAPLES,ITALY
关键词
antigen P-90; IgA; serodiagnosis; tuberculosis;
D O I
10.1378/chest.111.3.601
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Background: Serologic methods for diagnosis of tuberculosis have been widely investigated owing to their low cost and rapid technical execution. Sensitivity and specificity of different tests have been reported to be largely variable. Study objectives: To evaluate the IgA-mediated humoral immune response against the mycobacterial antigen P-90 as a tool for diagnosis of pulmonary tuberculosis. Participants: Eighty-eight patients with microbiologically confirmed diagnosis of pulmonary tuberculosis (32 with positive sputum smears and 56 with negative sputum smears), 28 patients with a definite nontuberculous lung disease, 12 subjects with healed tuberculosis, and 47 healthy volunteers (24 purified protein derivative negative and 23 positive). Measurements and results: Detection of anti-P-90 IgA was performed by enzyme-immunoassay. At a cutoff of 0.221 optical density, determined by a receiver operating characteristic curve, the overall sensitivity and specificity of the test were 70.4% and 91.9%, respectively. Patients with active tuberculosis showed significantly higher titers of anti-P-90 IgA compared with other groups (p<0.05). Conclusions: The evaluation of IgA-mediated humoral immune response against the antigen P-90 might constitute a useful tool for presumptive diagnosis of pulmonary tuberculosis.
引用
收藏
页码:601 / 605
页数:5
相关论文
共 30 条
  • [1] ALIFANO M, 1994, MICROBIOLOGICA, V17, P37
  • [2] IgA immune response against the mycobacterial antigen A60 in patients with active pulmonary tuberculosis
    Alifano, M
    Sofia, M
    Mormile, M
    Micco, A
    Mormile, AF
    DelPezzo, M
    Carratu, L
    [J]. RESPIRATION, 1996, 63 (05) : 292 - 297
  • [3] *AM THOR SOC, 1992, AM REV RESPIR DIS, V146, P1623
  • [4] TUBERCULOSIS IN PATIENTS WITH HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION
    BARNES, PF
    BLOCH, AB
    DAVIDSON, PT
    SNIDER, DE
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1991, 324 (23) : 1644 - 1650
  • [5] BENJAMIN RG, 1982, AM REV RESPIR DIS, V126, P1013
  • [6] RESURGENT TUBERCULOSIS IN NEW-YORK-CITY - HUMAN-IMMUNODEFICIENCY-VIRUS, HOMELESSNESS, AND THE DECLINE OF TUBERCULOSIS-CONTROL PROGRAMS
    BRUDNEY, K
    DOBKIN, J
    [J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 144 (04): : 745 - 749
  • [7] VALUE OF ELISA USING A60 ANTIGEN IN THE DIAGNOSIS OF ACTIVE PULMONARY TUBERCULOSIS
    CHARPIN, D
    HERBAULT, H
    GEVAUDAN, MJ
    SAADJIAN, M
    DEMICCO, P
    ARNAUD, A
    VERVLOET, D
    CHARPIN, J
    [J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1990, 142 (02): : 380 - 384
  • [8] CLANCY L, 1991, EUR RESPIR J, V4, P1288
  • [10] Das P K, 1989, Acta Leprol, V7 Suppl 1, P117