Chitosan oligosaccharides suppress production of nitric oxide in lipopolysaccharide-induced N9 murine microglial cells in vitro

被引：48

作者：

Wei, Peng ^{[1
,2
]}

Ma, Pan ^{[1
,2
]}

Xu, Qing-Song ^{[1
]}

Bai, Qun-Hua ^{[3
]}

Gu, Jian-Guo ^{[4
]}

Xi, Hao ^{[5
]}

Du, Yu-Guang ^{[1
]}

Yu, Chao ^{[3
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 110864, Peoples R China

[3] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China

[4] Tohoku Pharmaceut Univ, Div Regulatory Glycobiol, Inst Mol Biomembrane & Glycobiol, Sendai, Miyagi 9818558, Japan

[5] Sichuan Univ, Chengdu 610064, Peoples R China

来源：

GLYCOCONJUGATE JOURNAL | 2012年 / 29卷 / 5-6期

基金：

中国国家自然科学基金;

关键词：

Chitosan oligosaccharides; Microglial cell; Inflammation; MAPKs; NF-kappa B; AP-1; ACTIVATED PROTEIN-KINASES; MANNOSE RECEPTOR; C-FOS; EXPRESSION; INHIBITION; CHITOOLIGOSACCHARIDES; P38; OLIGOCHITOSAN; TRANSDUCTION; MODULATION;

D O I：

10.1007/s10719-012-9392-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Chitosan oligosaccharides (COS) have been reported to exert many biological activities, such as antioxidant, antitumor and anti-inflammatory effects. In the present study, we examined the effect of COS on nitric oxide (NO) production in LPS induced N9 microglial cells. Pretreatment with COS (50 similar to 200 mu g/ml) could markedly inhibit NO production by suppressing inducible nitric oxide synthase (iNOS) expression in activated microglial cells. Signal transduction studies showed that COS remarkably inhibited LPS-induced phosphorylation of p38 MAPK and ERK1/2. COS pretreatment could also inhibit the activation of both nuclear factor-kappa B (NF-kappa B) and activator protein-1 (AP-1). In conclusion, our results suggest that COS could suppress the production of NO in LPS-induced N9 microglial cells, mediated by p38 MAPK and ERK1/2 pathways.

引用

页码：285 / 295

页数：11

共 43 条

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