Determination of low-aliphatic aldehyde derivatizatives in human saliva using polymer monolith microextraction coupled to high-performance liquid chromatography

被引：83

作者：

Zhang, HJ ^{[1
]}

Huang, JF ^{[1
]}

Wang, H ^{[1
]}

Feng, YQ ^{[1
]}

机构：

[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 565卷 / 02期

基金：

中国国家自然科学基金;

关键词：

polymer monolith microextration; monolithic capillary; aldehydes; 2,4-dinitrophenylhydrazine; HPLC;

D O I：

10.1016/j.aca.2006.02.050

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, a polymer monolith microextraction (PMME) using a poly (methacrylic acid-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith in conjunction with high-performance liquid chromatography (HPLC) was developed for the determination of 2,4-dinitrophenylhydrazine (DNPH) derivatives of several aldehydes in human saliva. The conditions for the labeling reactions of aldehydes with DNPH and followed extraction of the derivatives were optimized. The precision, recovery and detection limits were evaluated with spiked saliva. The limits of detection ranged from 0.43 to 1.40 mu g/L. The inter-and intra-day relative standard deviations were less than 10%. The proposed method was successfully applied to the determination of aldehydes in saliva samples from a non-smoker, a passive smoker and a heavy smoker. (c) 2006 Elsevier B.V. All fights reserved.

引用

页码：129 / 135

页数：7

共 43 条

[1] High-performance liquid chromatography and capillary electrophoresis: Methodological challenges for the determination of biologically relevant low-aliphatic aldehydes in human saliva [J].