Microfluidic patterning of nanodisc lipid bilayers and multiplexed analysis of protein interaction

被引：28

作者：

Goluch, Edgar D. ^{[2
]}

Shaw, Andrew W. ^{[1
]}

Sligar, Stephen G. ^{[1
,3
,4
]}

Liu, Chang ^{[2
,5
,6
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[4] Univ Illinois, Coll Med, Urbana, IL 61801 USA

[5] Northwestern Univ, Dept Mech Engn, Evanston, IL 60208 USA

[6] Northwestern Univ, Dept Elect Engn & Comp Sci, Evanston, IL 60208 USA

来源：

LAB ON A CHIP | 2008年 / 8卷 / 10期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1039/b806733c

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a microfluidic method for precisely patterning lipid bilayers and a multiplexed assay to examine the interaction between the lipids and protein analytes. The lipids were packaged into nanoscale lipid bilayer particles known as Nanodiscs and delivered to surfaces using microfluidic channels. Two types of lipids were used in this study: biontinylated lipids and phosphoserine lipids. The deposition of biotinylated lipids on a glass surface was confirmed by attaching streptavidin coated quantum dots to the lipids, followed by fluorescent imaging. Using this multiplexed grid assay, we examined binding of annexin to phosphoserine lipids, and compared these results to similar analysis performed by surface plasmon resonance.

引用

页码：1723 / 1728

页数：6

共 35 条

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