Efficient transformation of Lactobacillus sake by electroporation

被引：99

作者：

Berthier, F ^{[1
]}

Zagorec, M ^{[1
]}

ChampomierVerges, M ^{[1
]}

Ehrlich, SD ^{[1
]}

MorelDeville, F ^{[1
]}

机构：

[1] INRA,LAB GENET MICROBIENNE,F-78352 JOUY EN JOSAS,FRANCE

来源：

MICROBIOLOGY-UK | 1996年 / 142卷

关键词：

Lactobacillus sake; electroporation; genetic transformation; vectors;

D O I：

10.1099/13500872-142-5-1273

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A procedure to transform intact Lactobacillus sake cells by electroporation was developed through a systematic examination of the effect of changes in various parameters on the transformation efficiency of Lact. sake strain 64F. The most critical factors were found to be the electrical parameters, the composition of washing and electroporation/storage solutions, and the presence of MgCl2 in the expression medium. Under optimal conditions transformation efficiencies up to 10(7) transformants (mu g supercoiled DNA)(-1) were obtained. The optimized procedure was successfully applied to other Lact. sake strains and consistently yielded from 10(4) to 10(7) transformants (mu g supercoiled DNA)(-1).

引用

页码：1273 / 1279

页数：7

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