Human Amniotic Fluid Stem Cells Can Integrate and Differentiate into Epithelial Lung Lineages

被引：175

作者：

Carraro, Gianni ^{[1
,2
]}

Perin, Laura ^{[1
,2
]}

Sedrakyan, Sargis ^{[1
,2
]}

Giuliani, Stefano ^{[1
,2
]}

Tiozzo, Caterina ^{[3
]}

Lee, Jooeun ^{[1
,2
]}

Turcatel, Gianluca ^{[1
,2
]}

De langhe, Stijn P. ^{[1
,2
]}

Driscoll, Barbara ^{[1
,2
]}

Bellusci, Saverio ^{[1
,2
]}

Minoo, Parviz ^{[3
]}

Atala, Anthony ^{[4
]}

De Filippo, Roger Edward ^{[1
,2
]}

Warburton, David ^{[1
,2
]}

机构：

[1] Childrens Hosp Los Angeles, Keck Sch Med, Saban Res Inst, Dev Biol Regenerat Med & Surg Program, Los Angeles, CA 90027 USA

[2] Sch Dent, Los Angeles, CA 90027 USA

[3] Univ So Calif, Keck Sch Med, Womens & Childrens Hosp, Los Angeles, CA 90033 USA

[4] Wake Forest Univ, Bowman Gray Sch Med, Wake Forest Inst Regenerat Med, Winston Salem, NC USA

来源：

STEM CELLS | 2008年 / 26卷 / 11期

关键词：

Amniotic fluid stem cells; Lung injury; Tissue repair; Regenerative medicine;

D O I：

10.1634/stemcells.2008-0090

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

A new source of stem cells has recently been isolated from amniotic fluid; these amniotic fluid stem cells have significant potential for regenerative medicine. These cells are multipotent, showing the ability to differentiate into cell types from each embryonic germ layer. We investigated the ability of human amniotic fluid stem cells (hAFSC) to integrate into murine lung and to differentiate into pulmonary lineages after injury. Using microinjection into cultured mouse embryonic lungs, hAFSC can integrate into the epithelium and express the early human differentiation marker thyroid transcription factor 1 (TTF1). In adult nude mice, following hyperoxia injury, vein-injected hAFSC localized in the distal lung and expressed both TTF1 and the type II pneumocyte marker surfactant protein C. Specific damage of Clara cells through naphthalene injury produced integration and differentiation of hAFSC at the bronchioalveolar and bronchial positions with expression of the specific Clara cell 10-kDa protein. These results illustrate the plasticity of hAFSC to respond in different ways to different types of lung damage by expressing specific alveolar versus bronchiolar epithelial cell lineage markers, depending on the type of injury to recipient lung. STEM CELLS 2008;26:2902-2911

引用

页码：2902 / 2911

页数：10

共 19 条

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[2]

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