Inhibition of peritoneal tumor cell growth and implantation in laparoscopic surgery in a rat model

被引:70
作者
Jacobi, CA [1 ]
Ordemann, J [1 ]
Bohm, B [1 ]
Zieren, HU [1 ]
Sabat, R [1 ]
Muller, JM [1 ]
机构
[1] HUMBOLDT UNIV BERLIN, DEPT CLIN IMMUNOL, D-10098 BERLIN, GERMANY
关键词
D O I
10.1016/S0002-9610(97)00099-8
中图分类号
R61 [外科手术学];
学科分类号
摘要
BACKGROUND: The pathogenesis of portsite recurrences after laparoscopic surgery is still unknown, and a generally accepted approach to prevent tumor implantation does not exist. METHODS: The effect of taurolidine and heparin on growth of colon adenocarcinoma DHD/K12/TRb was measured in vitro and in vivo, After incubation of the cells with heparin or taurolidine or both substances, cell kinetics were determined. In a rat model (n = 60), tumor cells were administered intraperitoneally, and pneumoperitoneum was established over 30 minutes, Rats received tumor cells, tumor cells + heparin, tumor cells + taurolidine, or tumor cells + taurolidine + heparin, RESULTS: In vitro, tumor cell growth decreased after incubation with taurolidine and taurolidine/heparin, In vivo, intraperitoneal tumor weight was lower in rats receiving heparin (298 +/- 155 mg) and taurolidine (149 +/- 247 mg) compared with the control group (596 +/- 278 mg) but even less when both substances were combined (21.5 +/- 36 mg). CONCLUSION: Heparin inhibits intraperitoneal tumor growth in vivo slightly, while taurolidine causes significant decrease of tumor cell growth in vitro as well as tumor take and intraperitoneal tumor growth in vivo. (C) 1997 by Excerpta Medica, Inc.
引用
收藏
页码:359 / 363
页数:5
相关论文
共 27 条
[1]   TAUROLIDINE, AN ANALOG OF THE AMINO-ACID TAURINE, SUPPRESSES INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR SYNTHESIS IN HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS [J].
BEDROSIAN, I ;
SOFIA, RD ;
WOLFF, SM ;
DINARELLO, CA .
CYTOKINE, 1991, 3 (06) :568-575
[3]  
BRODHAGE H, 1985, TAUROLIN NEUES KONZE, P38
[4]  
CASTRONOVO V, 1991, CANCER RES, V51, P5672
[5]  
CAVA A, 1990, EUR J SURG ONCOL, V16, P63
[6]  
CLAIR DG, 1993, SURGERY, V113, P355
[7]  
DROUARD F, 1991, NEW ENGL J MED, V325, P1316
[8]   ABDOMINAL-WALL RECURRENCE AFTER LAPAROSCOPIC-ASSISTED COLECTOMY FOR ADENOCARCINOMA OF THE COLON - REPORT OF A CASE [J].
FUSCO, MA ;
PALUZZI, MW .
DISEASES OF THE COLON & RECTUM, 1993, 36 (09) :858-861
[9]   PROTECTIVE EFFECTS OF HEPARIN AND OTHER SULFATED GLYCOSAMINOGLYCANS ON CRYSTAL ADHESION TO INJURED UROTHELIUM [J].
GILL, WB ;
JONES, KW ;
RUGGIERO, KJ .
JOURNAL OF UROLOGY, 1982, 127 (01) :152-154
[10]   INHIBITION OF PERITONEAL TUMOR-CELL IMPLANTATION - MODEL FOR LAPAROSCOPIC CANCER-SURGERY [J].
GOLDSTEIN, DS ;
LU, ML ;
HATTORI, T ;
RATLIFF, TL ;
LOUGHLIN, KR ;
KAVOUSSI, LR .
JOURNAL OF ENDOUROLOGY, 1993, 7 (03) :237-241