Chlorophyll a aggregates stabilized by a synthesized peptide

被引：4

作者：

Dudkowiak, A

Kusumi, T

Nakamura, C

Miyake, J

机构：

[1] MITI, AIST, Natl Inst Adv Interdisciplinary Res, Tsukuba, Ibaraki 3058562, Japan

[2] Poznan Univ Technol, Inst Phys, Div Mol Phys, PL-60965 Poznan, Poland

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 1999年 / 129卷 / 1-2期

关键词：

chlorophyll; histidine; peptides;

D O I：

10.1016/S1010-6030(99)00185-9

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The affinity of a synthesized amphiphilic peptide to bind Chi has been studied. It was shown that the peptide, which contains histidine, is able to stabilize the dimeric form of Chi in aqueous solution, making the pigment-peptide complex. The interaction between Chi and the amphiphilic peptide, without support of histidine, caused, the formation of higher pigment aggregates based on the hydrophobic interaction only. The dimer of Chl ligated to the peptide by the histidine residue showed that the absorption at 685 nm could serve as an in vitro model of the Chi a special dimer. The size and molecular weight of the Chi-peptide complex was estimated. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

收藏

页码：51 / 55

页数：5

相关论文

共 15 条

[1] AN INFRARED STUDY OF CHLOROPHYLL-CHLOROPHYLL AND CHLOROPHYLL-WATER INTERACTIONS [J].

BALLSCHMITER, K ;

KATZ, JJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1969, 91 (10) :2661-+

[2] Formation of stable polypeptide monolayers at interfaces: Controlling molecular conformation and orientation [J].

Boncheva, M ;

Vogel, H .

BIOPHYSICAL JOURNAL, 1997, 73 (02) :1056-1072

[3]

CAPRINO LA, 1972, J ORG CHEM, V37, P3404

[4] Interactions of chlorophyll a with synthesized peptide in aqueous solution [J].

Dudkowiak, A ;

Nakamura, C ;

Arai, T ;

Miyake, J .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1998, 45 (01) :43-50

[5] Mg coordination by amino acid side chains is not required for assembly and function of the special pair in bacterial photosynthetic reaction centers [J].

Goldsmith, JO ;

King, B ;

Boxer, SG .

BIOCHEMISTRY, 1996, 35 (07) :2421-2428

[6] PREPARATION AND PROPERTIES OF CHLOROPHYLL WATER-SOLUBLE MACROMOLECULAR COMPLEXES IN WATER - STABILIZATION OF CHLOROPHYLL AGGREGATES IN THE WATER-SOLUBLE MACROMOLECULE [J].

INAMURA, I ;

OCHIAI, H ;

TOKI, K ;

WATANABE, S ;

HIKINO, S ;

ARAKI, T .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1983, 38 (01) :37-44

[7] RAMAN-SPECTRA OF CHLOROPHYLL FORMS [J].

KOYAMA, Y ;

UMEMOTO, Y ;

AKAMATSU, A ;

UEHARA, K ;

TANAKA, M .

JOURNAL OF MOLECULAR STRUCTURE, 1986, 146 :273-287

[8] The interactions between bacteriochlorophyll c and amphiphilic peptides [J].

Miyake, J ;

Kusumi, T ;

Dudkowiak, A ;

Goc, J ;

Frackowiak, D .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 1998, 116 (02) :147-151

[9] PREPARATION AND PROPERTIES OF THE WATER-SOLUBLE CHLOROPHYLL-BOVINE SERUM-ALBUMIN COMPLEXES [J].

SHIBATA, H ;

OCHIAI, H ;

KAWASHIMA, T ;

OKAMOTO, T ;

INAMURA, I .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 852 (2-3) :175-182

[10] CALCULATION OF ELECTRONIC-SPECTRA OF CHLOROPHYLL-A-WATER AND BACTERIOCHLOROPHYLL-A-WATER ADDUCTS [J].

SHIPMAN, LL ;

KATZ, JJ .

JOURNAL OF PHYSICAL CHEMISTRY, 1977, 81 (06) :577-581