High-yield synthesis of N-acetyllactosamine by regioselective transglycosylation

被引：46

作者：

Vetere, A ^{[1
]}

Paoletti, S ^{[1
]}

机构：

[1] UNIV SASSARI,FAC MED & CHIRURG,IST CHIM BIOL,I-07100 SASSARI,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 219卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.0172

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of N-acetyllaetosamine (D-Galp beta 1-4D-GlepNAc) with very low contamination of its isomer N-acetylallolactosamine (D-Galp beta 1-6D-GlepNAc) was obtained by use of regioselective transglycosylation activity of beta-galactosidase from Bacillus circulans using lactose as thr donor of D-Galp and D-GlepNAc as the acceptor. The reaction was conducted at 15 degrees C and at pH 5.0. The incubation time was considerably reduced and the yield improved 100% with respect to the best results so far described in tile literature. (C) 1996 Academic Press Inc.

引用

页码：6 / 13

页数：8

共 30 条

[1] ENZYMIC-SYNTHESIS OF DISACCHARIDES BY USE OF THE REVERSED HYDROLYSIS ACTIVITY OF BETA-D-GALACTOSIDASES [J].