Enzymatic degradation of poly(3-hydroxybutyrate) by a commercial lipase

被引：28

作者：

Alejandra, Rodriguez-Contreras ^{[1
]}

Margarita, Calafell-Monfort ^{[2
]}

Maria Soledad, Marques-Calvo ^{[1
]}

机构：

[1] Tech Univ Catalonia, Dept Opt & Optometry, Barcelona 08222, Spain

[2] Tech Univ Catalonia, Dept Chem Engn, Barcelona 08222, Spain

来源：

POLYMER DEGRADATION AND STABILITY | 2012年 / 97卷 / 11期

关键词：

Polyhydroxyalkanoates; Lipase; Poly(3-hydroxybutyrate); Enzymatic degradation; POLYHYDROXYALKANOATES; POLYHYDROXYBUTYRATE; COPOLYMERS; POLYESTERS; BACTERIAL; RECOVERY; GRANULES; ACID); PHB;

D O I：

10.1016/j.polymdegradstab.2012.07.042

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 [高分子化学与物理];

摘要：

Polyesters such as poly(3-hydroxybutyrate) (PHB) have attracted commercial and academic interest as new biotechnological materials. In previous studies it was confirmed that two commercial lipases hydrolyzed the ester bonds from the 3HB fractions of P(3HB-co-4HB) copolymer. In this study, one of the previously used commercial lipases has been used for the enzymatic degradation of PHB homopolymer obtained via fermentation with Cupriavidus necator in order to obtain low molecular mass polymer. The results confirmed the enzymatic reaction of the used lipase with this PHB and show a controlled decrease of the molecular mass from 300,000 Da-4000 Da. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：2473 / 2476

页数：4

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