p38 mitogen-activated protein kinase regulates IL-8 expression in human pulmonary vascular endothelial cells

被引：42

作者：

Hashimoto, S ^{[1
]}

Matsumoto, K ^{[1
]}

Gon, Y ^{[1
]}

Maruoka, S ^{[1
]}

Takeshita, I ^{[1
]}

Hayashi, S ^{[1
]}

Koura, T ^{[1
]}

Kujime, K ^{[1
]}

Horie, T ^{[1
]}

机构：

[1] Nihon Univ, Sch Med, Dept Internal Med 1, Itabashi Ku, Tokyo 1738610, Japan

来源：

EUROPEAN RESPIRATORY JOURNAL | 1999年 / 13卷 / 06期

关键词：

acute lung injury; interleukin-8; p3S MAP kinase; pulmonary vascular endothelium;

D O I：

10.1034/j.1399-3003.1999.13f21.x

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

The aim of this study was to examine the role of p38 mitogen-activated protein (MAP) kinase in interleukin (IL)-8 expression in tumour necrosis factor (TNF)-alpha- and IL-1 alpha-stimulated human pulmonary vascular endothelial cells. To this end, the phosphorylation and activation of p38 MAP kinase and the effect of SE 203580, a specific inhibitor of p38 MAP kinase activity, on p38 MAP kinase activity and IL-8 expression in TNF-alpha- and IL-1 alpha-stimulated human pulmonary vascular endothelial cells were examined. TNF-alpha- and IL-1 alpha- induced phosphorylation and activation of p38 MAP kinase and IL-8 expression in human pulmonary endothelial cells. Inhibition of TNF-alpha- and IL-1 alpha-induced p38 MAP kinase activity by SE 203580 inhibited TNF-alpha- and IL-1 alpha-induced IL-8 protein production as well as IL-8 messenger ribonucleic acid (mRNA) expression, indicating that SE 203580 was effective at the transcriptional level. These results indicate that p38 mitogen-activated protein kinase plays an important role in the tumour necrosis factor-alpha- and interleukin-1 or-activated signalling pathway which regulates interleukin-8 expression in human pulmonary vascular endothelial cells.

引用

页码：1357 / 1364

页数：8

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