Porin from Pseudomonas aeruginosa induces apoptosis in an epithelial cell line derived from rat seminal vesicles

被引：46

作者：

Buommino, E

Morelli, F

Metafora, S

Rossano, F

Perfetto, B

Baroni, A

Tufano, MA

机构：

[1] Univ Naples 2, Sch Med, Ist Microbiol, I-80138 Naples, Italy

[2] Univ Naples 2, Sch Med, Ist Clin Dennosilfilopat, I-80138 Naples, Italy

[3] CNR, Int Inst Genet & Biophys, I-80125 Naples, Italy

[4] CNR, Serv Tecnol Biomol, Area Ric, I-80125 Naples, Italy

来源：

INFECTION AND IMMUNITY | 1999年 / 67卷 / 09期

关键词：

D O I：

10.1128/IAI.67.9.4794-4800.1999

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Micromolar concentrations of porin, purified from the outer membranes of Pseudomonas aeruginosa, induced in vitro the classic morphological and biochemical signs of apoptosis in an epithelial cell line (SVC1) derived from the rat seminal vesicle secretory epithelium. The programmed cell death (PCD) was p53 independent and associated with significant decrease of bcl-2 expression, a marked increase of c-myc transcriptional activity, and an absence of the mRNA coding for tissue transglutaminase. The Ca2+ influx, caused by the porin treatment of SVC1 cells, appears to play an important role in the triggering of apoptosis in our biological model. The possibility that the porin property of inducing PCD plays a role in the infertility of individuals chronically infected by gram-negative bacteria is discussed.

引用

页码：4794 / 4800

页数：7

共 48 条

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