The mediation of veratryl alcohol in oxidations promoted by lignin peroxidase: the lifetime of veratryl alcohol radical cation

被引：20

作者：

Baciocchi, E

Bietti, M

Gerini, MF

Lanzalunga, O

机构：

[1] Univ Roma La Sapienza, Dipartmento Chim, I-00185 Rome, Italy

[2] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, I-00133 Rome, Italy

[3] Univ Roma La Sapienza, Ctr CNR Studio Meccanismi Reaz, I-00185 Rome, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 293卷 / 02期

关键词：

veratryl alcohol; radical cation; lignin degradation; lignin peroxidase; diffusible redox mediator; stopped-flow;

D O I：

10.1016/S0006-291X(02)00306-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics of decay of veratryl alcohol radical cation, generated by cerium(IV) ammonium nitrate induced oxidation of veratryl alcohol, have been followed spectrophotometrically in a stopped-flow apparatus. In acidic aqueous acetonitrile the radical cation was found to decay by a first-order process, due to deprotonation from the a-carbon leading to an a-hydroxybenzyl radical with the rate constant of 17.1 +/- 0.5 s(-1). This value is in full agreement with those obtained by pulse radiolysis studies but much lower than the value (1.2 x 10(3)s(-1)) indirectly determined by EPR experiments. The implications of these results with respect to the possible role of veratryl alcohol as a mediator in the oxidative biodegradation of lignin catalysed by lignin peroxidase are discussed. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：832 / 835

页数：4

共 22 条

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