Identification, tissue localisation and physiological effect in vitro of a neuroendocrine peptide identical to a dipteran Leu-callatostatin in the codling moth Cydia pomonella (Tortricidae: Lepidoptera)

被引:59
作者
Duve, H
Johnsen, AH
Maestro, JL
Scott, AG
Crook, N
Winstanley, D
Thorpe, A
机构
[1] UNIV LONDON QUEEN MARY & WESTFIELD COLL,SCH BIOL SCI,LONDON E1 4NS,ENGLAND
[2] STATE UNIV HOSP,DEPT CLIN CHEM,DK-2100 COPENHAGEN,DENMARK
[3] HORT RES INT,WELLESBOURNE CV35 9EF,WARWICK,ENGLAND
关键词
Leu-callatostatins; callatostatins; allatostatins; neuropeptides; peptide purification; immunocytochemistry; myoinhibition; Codling moth; Cydia pomonella (Tortricidae: Lepidoptera) (Insecta);
D O I
10.1007/s004410050853
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A neuroendocrine peptide of the Leu-callatostatin family, LPVYNFGL-NH2, has been isolated from tissue extracts of 5th instar larvae of the codling moth, Cydia pomonella (Lepidoptera). It is identical to a peptide previously isolated from the blowfly, Calliphora vomitoria (Diptera). The distribution of this peptide within the tissues of C. pomonella has been mapped by immunocytochemistry using antisera raised against LPVYNFGL-NH2. Midgut endocrine cells contain Leu-callatostatin immunoreactivity, as do several paired Leu-callatostatin neurones in the brain and ventral nerve cord. Within the visceral nervous system, the frontal ganglion contains four Leu-callatostatin neurones. Axons from these cells combine with others originating from neurones in the brain and project within the nervi cardiostomatogastrici to innervate the tissues of the foregut. In particular, the oesophageal valve has a prominent ring of Leu-callatostatin-immunoreactive fibres. The synthetic peptide, LPVYNFGL-NH2, has a potent reversible inhibitory effect in vitro on all visible forms of spontaneous contractile activity of the foregut, including closure of the oesophageal valve. Complete myoinhibition is observed at peptide concentrations from 10(-10) to 10(-16) M. These results, in conjunction with the results of similar studies on cockroaches, crickets and flies, suggest that the Leu-callatostatins are a ubiquitous family of insect neuroendocrine peptides with an important role in the control of gut motility.
引用
收藏
页码:73 / 83
页数:11
相关论文
共 32 条
[1]   ALLATOSTATIC NEUROPEPTIDES FROM THE COCKROACH BLATTELLA-GERMANICA (L) (DICTYOPTERA, BLATTELLIDAE) - IDENTIFICATION, IMMUNOLOCALIZATION AND ACTIVITY [J].
BELLES, X ;
MAESTRO, JL ;
PIULACHS, MD ;
JOHNSEN, AH ;
DUVE, H ;
THORPE, A .
REGULATORY PEPTIDES, 1994, 53 (03) :237-247
[2]   MOLECULAR-CLONING OF THE GENE FOR THE ALLATOSTATIN FAMILY OF NEUROPEPTIDES FROM THE COCKROACH DIPLOPTERA-PUNCTATA [J].
DONLY, BC ;
DING, Q ;
TOBE, SS ;
BENDENA, WG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (19) :8807-8811
[3]   Identification of the dipteran Leu-callatostatin peptide family: The pattern of precursor processing revealed by isolation studies in Calliphora vomitoria [J].
Duve, H ;
Johnsen, AH ;
Maestro, JL ;
Scott, AG ;
East, PD ;
Thorpe, A .
REGULATORY PEPTIDES, 1996, 67 (01) :11-19
[4]   CALLATOSTATINS - NEUROPEPTIDES FROM THE BLOWFLY CALLIPHORA-VOMITORIA WITH SEQUENCE HOMOLOGY TO COCKROACH ALLATOSTATINS [J].
DUVE, H ;
JOHNSEN, AH ;
SCOTT, AG ;
YU, CG ;
YAGI, KJ ;
TOBE, SS ;
THORPE, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (06) :2456-2460
[5]  
DUVE H, 1994, J BIOL CHEM, V269, P21059
[6]   INNERVATION OF THE FOREGUT OF THE COCKROACH LEUCOPHAEA-MADERAE AND INHIBITION OF SPONTANEOUS CONTRACTILE ACTIVITY BY CALLATOSTATIN NEUROPEPTIDES [J].
DUVE, H ;
WREN, P ;
THORPE, A .
PHYSIOLOGICAL ENTOMOLOGY, 1995, 20 (01) :33-44
[7]   DISTRIBUTION AND FUNCTIONAL-SIGNIFICANCE OF LEU-CALLATOSTATINS IN THE BLOWFLY CALLIPHORA-VOMITORIA [J].
DUVE, H ;
THORPE, A .
CELL AND TISSUE RESEARCH, 1994, 276 (02) :367-379
[9]   ISOLATION, IDENTIFICATION AND FUNCTIONAL-SIGNIFICANCE OF [HYP(2)]MET-CALLATOSTATIN AND DES GLY-PRO MET-CALLATOSTATIN, 2 FURTHER POSTTRANSLATIONAL MODIFICATIONS OF THE BLOWFLY NEUROPEPTIDE MET-CALLATOSTATIN [J].
DUVE, H ;
JOHNSEN, AH ;
SCOTT, AG ;
THORPE, A .
REGULATORY PEPTIDES, 1995, 57 (03) :237-245
[10]  
DUVE H, 1996, IN PRESS SEB SEM SER