Energetics of Photosystem II charge recombination in Acaryochloris marina studied by thermoluminescence and flash-induced chlorophyll fluorescence measurements

被引:20
作者
Cser, Krisztian [1 ]
Deak, Zsuzsanna [1 ]
Telfer, Alison [2 ]
Barber, James [2 ]
Vass, Imre [1 ]
机构
[1] Biol Res Ctr, Inst Plant Biol, H-6726 Szeged, Hungary
[2] Univ London Imperial Coll Sci Technol & Med, Div Mol Biosci, London SW7 2AZ, England
基金
英国生物技术与生命科学研究理事会;
关键词
Photosystem II; Charge separation; Electron transfer; Acaryochloris marina; Chlorophyll d; Thermoluminescence; Fluorescence;
D O I
10.1007/s11120-008-9373-3
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We studied the charge recombination characteristics of Photosystem II (PSII) redox components in whole cells of the chlorophyll (Chl) d-dominated cyanobacterium, Acaryochloris marina, by flash-induced chlorophyll fluorescence and thermoluminescence measurements. Flash-induced chlorophyll fluorescence decay was retarded in the mu s and ms time ranges and accelerated in the s time range in Acaryochloris marina relative to that in the Chl a-containing cyanobacterium, Synechocystis PCC 6803. In the presence of 3-(3,4-dichlorophenyl)-1, 1-dimethylurea, which blocks the Q(B) site, the relaxation of fluorescence decay arising from S(2)Q(A)(-) recombination was somewhat faster in Acaryochloris marina than in Synechocystis PCC 6803. Thermoluminescence intensity of the so called B band, arising from the recombination of the S(2)Q(B)(-) charge separated state, was enhanced significantly (2.5 fold) on the basis of equal amounts of PSII in Acaryochloris marina as compared with Synechocystis 6803. Our data show that the energetics of charge recombination is modified in Acaryochloris marina leading to a similar to 15 meV decrease of the free energy gap between the Q(A) and QB acceptors. In addition, the total free energy gap between the ground state and the excited state of the reaction center chlorophyll is at least similar to 25-30 meV smaller in Acaryochloris marina, suggesting that the primary donor species cannot consist entirely of Chl a in Acaryochloris marina, and there is a contribution from Chl d as well.
引用
收藏
页码:131 / 140
页数:10
相关论文
共 45 条
[1]   CHANGES IN COMPOSITION OF MEMBRANE-PROTEINS ACCOMPANYING THE REGULATION OF PS-I/PS-II STOICHIOMETRY OBSERVED WITH SYNECHOCYSTIS PCC-6803 [J].
AIZAWA, K ;
SHIMIZU, T ;
HIYAMA, T ;
SATOH, K ;
NAKAMURA, Y ;
FUJITA, Y .
PHOTOSYNTHESIS RESEARCH, 1992, 32 (02) :131-138
[2]   Quest for minor but key chlorophyll molecules in photosynthetic reaction centers -: unusual pigment composition in the reaction centers of the chlorophyll d-dominated cyanobacterium Acaryochloris marina [J].
Akiyama, M ;
Miyashita, H ;
Kise, H ;
Watanabe, T ;
Mimuro, M ;
Miyachi, S ;
Kobayashi, M .
PHOTOSYNTHESIS RESEARCH, 2002, 74 (02) :97-107
[3]   The function of D1-H332 in Photosystem II electron transport studied by thermoluminescence and chlorophyll fluorescence in site-directed mutants of Synechocystis 6803 [J].
Allahverdiyeva, Y ;
Deák, Z ;
Szilárd, A ;
Diner, BA ;
Nixon, PJ ;
Vass, I .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2004, 271 (17) :3523-3532
[4]   CHLORORESPIRATION REVISITED - MITOCHONDRIAL-PLASTID INTERACTIONS IN CHLAMYDOMONAS [J].
BENNOUN, P .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1994, 1186 (1-2) :59-66
[5]   The nature of the photosystem II reaction centre in the chlorophyll d-containing prokaryote, Acaryochloris marina [J].
Chen, M ;
Telfer, A ;
Lin, S ;
Pascal, A ;
Larkum, AWD ;
Barber, J ;
Blankenship, RE .
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2005, 4 (12) :1060-1064
[6]   AMINO-ACID-RESIDUES THAT INFLUENCE THE BINDING OF MANGANESE OR CALCIUM TO PHOTOSYSTEM-II .2. THE CARBOXY-TERMINAL DOMAIN OF THE D1 POLYPEPTIDE [J].
CHU, HA ;
NGUYEN, AP ;
DEBUS, RJ .
BIOCHEMISTRY, 1995, 34 (17) :5859-5882
[7]  
CREUZET S, 1990, Z NATURFORSCH C, V45, P436
[8]   THE ELECTROCHEMICAL DOMAIN OF PHOTOSYNTHESIS [J].
CROFTS, AR ;
WRAIGHT, CA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 726 (03) :149-185
[9]   Radiative and non-radiative charge recombination pathways in Photosystem II studied by thermoluminescence and chlorophyll fluorescence in the cyanobacterium Synechocystis 6803 [J].
Cser, Krisztian ;
Vass, Imre .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2007, 1767 (03) :233-243
[10]   Kinetics of electron transfer from QA to QB in photosystem II [J].
de Wijn, R ;
van Gorkom, HJ .
BIOCHEMISTRY, 2001, 40 (39) :11912-11922