Elemental bio-imaging of melanoma in lymph node biopsies

被引：45

作者：

Hare, Dominic ^{[1
]}

Burger, Fiona ^{[1
]}

Austin, Christine ^{[1
]}

Fryer, Fred ^{[2
]}

Grimm, Rudolf ^{[3
]}

Reedy, Brian ^{[1
]}

Scolyer, Richard A. ^{[4
,5
]}

Thompson, John F. ^{[4
,5
]}

Doble, Philip ^{[1
]}

机构：

[1] Univ Technol Sydney, Dept Chem & Forens Sci, Elemental Bioimaging Facil, Ultimo, NSW 2007, Australia

[2] Agilent Technol Australia, N Ryde, NSW, Australia

[3] Agilent Technol, Santa Clara, CA USA

[4] Royal Prince Alfred & Mater Hosp, Sydney Melanoma Unit, Sydney, NSW, Australia

[5] Royal Prince Alfred & Mater Hosp, Melanoma Inst Australia, Sydney, NSW, Australia

来源：

ANALYST | 2009年 / 134卷 / 03期

关键词：

PLASMA-MASS SPECTROMETRY; LASER-ABLATION; THIN-SECTIONS; MALIGNANT-MELANOMA; BIOLOGICAL SAMPLES; BRAIN-TUMOR; ZINC; ZN; CU; MS;

D O I：

10.1039/b812745j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The spatial distribution of trace elements in human lymph nodes partially infiltrated by melanoma cells was determined by elemental bio-imaging. Imaging of P-31 within the nodal capsule and normal lymph node tissue showed a clear demarcation of the tumour boundary, with a significant decrease in relative P-31 concentration within the tumour. The location of the tumour boundary was confirmed by haematoxylin and eosin staining of serial sections and observation by light microscopy. Further enhancement of the tumour boundary was achieved by imaging the P-31/S-34 ratio. P-31/Zn-66 ratio images showed a decreasing ratio beyond the tumour boundary that extended into peritumour normal lymph node tissue.

引用

页码：450 / 453

页数：4

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