Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development

被引：52

作者：

Horsfield, J

Ramachandran, A

Reuter, K

LaVallie, E

Collins-Racie, L

Crosier, K

Crosier, P ^{[1
]}

机构：

[1] Univ Auckland, Dept Mol Med & Pathol, Auckland 1, New Zealand

[2] Wyeth Ayerst Res, Genet Inst, Cambridge, MA 02140 USA

来源：

MECHANISMS OF DEVELOPMENT | 2002年 / 115卷 / 1-2期

关键词：

cadherin-17; CDH17; LI-cadherin; cell adhesion; kidney; pronephric duct; pronephros; mesonephros; zebrafish;

D O I：

10.1016/S0925-4773(02)00094-1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have isolated a zebrafish cadherin that is orthologous to human LI-cadherin (CDH17). Zebrafish cdh17 is expressed exclusively in the pronephric ducts during embryogenesis, and in the mesonephros during larval development and adulthood. Like its mammalian ortholog, cdh17 is also expressed in liver and intestine in adult zebrafish. We show that cdh17-positive mesodermal cells do not contribute to the hematopoietic system. Consistent with a cell adhesion role for Cdh17, depletion of Cdh17 function using antisense morpholino oligonucleotides compromised cell cohesion during pronephric duct formation. Our results indicate that Cdh17 is necessary for maintaining the integrity of the pronephric ducts during zebrafish embryogenesis. This finding contrasts with the role of mammalian CDH17. which does not appear to be involved in nephric development. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：15 / 26

页数：12

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