Immunolocalization of GLUTX1 in the testis and to specific brain areas and vasopressin-containing neurons

被引:60
作者
Ibberson, M
Riederer, BM
Uldry, M
Guhl, B
Roth, J
Thorens, B
机构
[1] Univ Lausanne, Inst Pharmacol & Toxicol, CH-1005 Lausanne, Switzerland
[2] Univ Lausanne, Inst Cell Biol & Morphol, CH-1005 Lausanne, Switzerland
[3] Univ Zurich, Dept Pathol, Div Cell & Mol Pathol, CH-8091 Zurich, Switzerland
关键词
D O I
10.1210/en.143.1.276
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
GLUTX1 or GLUT8 is a newly characterized glucose transporter isoform. that is expressed at high levels in the testis and brain and at lower levels in several other tissues. Its expression was mapped in the testis and brain by using specific antibodies. In the testis, immunoreactivity was expressed in differentiating spermatocytes of type 1 stage but undetectable in mature spermatozoa. In the brain, GLUTX1 distribution was selective and localized to a variety of structures, mainly archi- and paleocortex. It was found in hippocampal and dentate gyrus neurons as well as amygdala and primary olfactory cortex. In these neurons, its location was close to the plasma membrane of cell bodies and sometimes in proximal dendrites. High GLUTX1 levels were detected in the hypothalamus, supraoptic nucleus, median eminence, and the posterior pituitary. Neurons of these areas synthesize and secrete vasopressin and oxytocin. As shown by double immunofluorescence microscopy and immunogold labeling, GLUTX1 was expressed only in vasopressin neurons. By immunogold labeling of ultrathin cryosections microscopy, GLUTX1 was identified in dense core vesicles of synaptic nerve endings of the supraoptic nucleus and secretory granules of the vasopressin positive neurons. This localization suggests an involvement of GLUTX1 both in specific neuron function and endocrine mechanisms.
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收藏
页码:276 / 284
页数:9
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