Long-Term Survival and Bipotent Terminal Differentiation of Human Mesenchymal Stem Cells (hMSC) in Combination With a Commercially Available Three-Dimensional Collagen Scaffold

被引:58
作者
Neuss, S. [1 ,2 ]
Stainforth, R. [1 ]
Salber, J. [3 ]
Schenck, P. [4 ]
Bovi, M. [5 ]
Knuechel, R. [1 ]
Perez-Bouza, A. [1 ]
机构
[1] Univ Aachen, Rhein Westfal TH Aachen, Inst Pathol, D-52074 Aachen, Germany
[2] Univ Aachen, Rhein Westfal TH Aachen, Interdisciplinary Ctr Clin Res, IZKF Biomat, D-52074 Aachen, Germany
[3] Univ Aachen, Rhein Westfal TH Aachen, Dept Text & Macromol Chem, D-52074 Aachen, Germany
[4] Dr Suwelack Skin & Hlth Care AG, D-48727 Billerbeck, Germany
[5] Univ Aachen, Rhein Westfal TH Aachen, Electron Microscop Facil, D-52074 Aachen, Germany
关键词
Mesenchymal stem cells; Autologous cells; Adipose tissue engineering; Bone tissue engineering; Collagen; Electron microscopy;
D O I
10.3727/096368908786576462
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Researchers working in the field of tissue engineering ideally combine autologous cells and biocompatible scaffolds to replace defect tissues/organs. Due to their differentiation capacity, mesenchym-derived stem cells, such as human mesenchymal stem cells (hMSC), are a promising autologous cell source for the treatment of human diseases. As natural precursors for mesenchymal tissues, hMSC are particularly suitable for bone, cartilage, and adipose tissue replacement. In this study a detailed histological and ultrastructural analysis of long-term cultured and terminally differentiated hMSC on 3D collagen scaffolds was performed. Standardized 2D differentiation protocols for hMSC into adipocytes and osteoblasts were adapted for long-term 3D in vitro cultures in porous collagen matrices. After a 50-day culture period, large numbers of mature adipocytes and osteoblasts were clearly identifiable within the scaffolds. The adipocytes exhibited membrane free lipid vacuoles. The osteoblasts were arranged in close association with hydroxyapatite crystals, which were deposited on the surrounding fibers. The collagen matrix was remodeled and adopted a contracted and curved form. Human MSC survive long-term culture within these scaffolds and could be terminally differentiated into adipocytes and osteoblasts. Thus, the combination of hMSC and this particular collagen scaffold is a possible candidate for bone and adipose tissue replacement strategies.
引用
收藏
页码:977 / 986
页数:10
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