Construction and characterization of a sheep BAC library of three genome equivalents

被引：66

作者：

Vaiman, D ^{[1
]}

Billault, A

Tabet-Aoul, K

Schibler, L

Vilette, D

Oustry-Vaiman, A

Soravito, C

Cribiu, EP

机构：

[1] INRA, Lab Genet Biochim & Cytogenet, Dept Genet Anim, F-78352 Jouy En Josas, France

[2] Fdn Jean Dausset, Ctr Etud Polymorphisme Humain, F-75010 Paris, France

[3] INRA, Pathol Infect & Immunol Lab, F-37380 Nouzilly, France

来源：

MAMMALIAN GENOME | 1999年 / 10卷 / 06期

关键词：

D O I：

10.1007/s003359901049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sheep BAC library of over three genome equivalents was constructed and arrayed in superpools and row, column, and plate pools. The library contains 90,000 clones distributed in 39 superpools. The average insert size was estimated at 123 kb. The library was screened by PCR with 77 primer pairs corresponding to ovine microsatellites distributed throughout the genome. The probability of finding a random sequence in the library could be estimated at 0.96.

引用

页码：585 / 587

页数：3

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