Illegitimate recombination induced by overproduction of DnaB helicase in Escherichia coli

被引：12

作者：

Yamashita, T ^{[1
]}

Hanada, K ^{[1
]}

Iwasaki, M ^{[1
]}

Yamaguchi, I ^{[1
]}

Ikeda, H ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 15期

关键词：

D O I：

10.1128/JB.181.15.4549-4553.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Illegitimate recombination that usually takes place at a low frequency is greatly enhanced by treatment with DNA-damaging agents. It is thought that DNA double-strand breaks: induced by this DNA damage are important for initiation of illegitimate recombination. Here we show that illegitimate recombination is enhanced by overexpression of the DnaB protein in Escherichia coli. The recombination enhanced by DnaB overexpression occurred between short regions of homology. We propose a model for the initiation of illegitimate recombination in which DnaB overexpression may excessively unwind DNA at replication forks and induce double-strand breaks, resulting in illegitimate recombination. The defect in RecQ has a synergistic effect on the increased illegitimate recombination in cells containing the overproduced DnaB protein, implying that DnaB works in the same pathway as RecQ does but that they work at different steps.

引用

页码：4549 / 4553

页数：5

共 29 条

[1] ON THE FORMATION OF SPONTANEOUS DELETIONS - THE IMPORTANCE OF SHORT SEQUENCE HOMOLOGIES IN THE GENERATION OF LARGE DELETIONS [J].