Indirect competitive immunoassay for trichlorophenol determination - Rational evaluation of the competitor heterology effect

被引：48

作者：

Galve, R ^{[1
]}

Sanchez-Baeza, F ^{[1
]}

Camps, F ^{[1
]}

Marco, MP ^{[1
]}

机构：

[1] CSIC, IIQAB, Dept Biol Organ Chem, Barcelona 08034, Spain

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 452卷 / 02期

关键词：

immunoassay; chlorophenol; hapten design; molecular modeling; heterology;

D O I：

10.1016/S0003-2670(01)01452-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An improved competitive indirect immunoassay for the detection of 2,4,6-trichlorophenol (2,4,6-TCP) has been developed and optimized by preparing heterologous haptens that have been evaluated as coating antigens. The relation between the degree of heterology and immunoassay detectability has been investigated according to the geometric and electronic distribution similarities between the haptens and the analyte using molecular modeling tools. The assay has been characterized according to different physicochemical parameters such as the incubation time, the ionic strength, the effect of detergents and the pH. The resulting assay has an IC50 of 1.44 mug l(-1) and a limit of detection (LOD) of 0.2 mug l(-1) and it shows a good accuracy and suitability to analyze trichlorophenol in drinking water. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：191 / 206

页数：16

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