Growth hormone (GH) and prolactin receptors in human peripheral blood mononuclear cells: Relation with age and GH-binding protein

被引:53
作者
Bresson, JL
Jeay, S
Gagnerault, MC
Kayser, C
Beressi, N
Wu, ZD
Kinet, S
Dardenne, M
Postel-Vinay, MC
机构
[1] Hop Necker Enfants Malad, INSERM, Unite 344, F-75730 Paris 15, France
[2] Hop Necker Enfants Malad, Ctr Invest Clin, F-75730 Paris 15, France
[3] Univ Paris 05, CNRS, URA 1461, F-75730 Paris, France
[4] Univ Liege, Lab Mol Biol & Genet Engn, B-4000 Liege, Belgium
[5] Univ Munich, Dept Med, D-80336 Munich, Germany
关键词
D O I
10.1210/en.140.7.3203
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
GH receptors (GHRs) and PRL receptors (PRLRs) were studied in human peripheral blood mononuclear cells (PBMC) using flow cytometry, biotinylated anti-GH receptor monoclonal antibody 10B8, and biotinylated human PRL. Variations of GHR and PRLR expression and the relationship of plasma GHBP and GH receptor in PBMC subsets were examined as a function of age and sex. By double immunofluorescence staining, we show that about 30% of total cells express GH receptors, with a low expression in T cells, whereas almost all B cells and monocytes are GH receptor positive. Four age groups were defined among the 64 normal volunteers, aged 12 to 85 yr, who were included in the study. The percentage of PBMC expressing GH receptors is significantly lower in group 2 (20-40 yr) than in group 1 (12-20 yr) and group 4 (>60 yr). In T cells, monocytes and B cells, no significant changes are detected in either the percentage of GH receptor positive cells or in the GH receptor level per cell. The level of PRLRs expressed in PBMC is significantly higher in age group 2 than in age group 4. A negative correlation is observed between plasma GHBP and the percentage of PBMC expressing GH receptors. These results suggest that regulation of GH receptors in lymphocytes and in other target cells could be different.
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页码:3203 / 3209
页数:7
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