4-chlorophenol degradation by chloroperoxidase from Caldariomyces fumago -: Formation of insoluble products

被引：12

作者：

La Rotta, CE ^{[1
]}

Bon, EPS ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Quim, Lab Tecnol Enzimat, BR-21949900 Rio De Janeiro, Brazil

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2002年 / 98卷 / 1-9期

关键词：

Caldariomyces fumago; chloroperoxidase; 4-chlorophenol degradation; polymerization reactions; industrial effluents treatment;

D O I：

10.1385/ABAB:98-100:1-9:191

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study investigated the degradation of 4-chlorophenol (4-CP) by Caldariomyces fumago chloroperoxidase (CPO). Enzymatic oxidations were studied in reaction mixtures at pH 3.0, 4.0, and 6.0 in the presence and absence of Cl- containing 3.5 IU of CPO and 4-CP and hydrogen peroxide concentrations within the range of 0.5-50 and 0.005-50 mM, respectively. Distinct patterns of products regarding color, concentration, and solubility were observed. Reaction mixtures at pH 6.0 containing 3.5 IU of CPO and 5.0 mM 4-CP and H2O2 (1:1 stoichiometry) showed the highest 4-CP removal of 95% and the highest formation of a dark precipitate.

引用

页码：191 / 203

页数：13

共 28 条

[1] CHARACTERIZATION OF REACTION-PRODUCTS FROM THE ENZYME-CATALYZED OXIDATION OF PHENOLIC POLLUTANTS [J].

AITKEN, MD ;

MASSEY, IJ ;

CHEN, TP ;

HECK, PE .

WATER RESEARCH, 1994, 28 (09) :1879-1889

[2] DEPHENOLIZATION OF INDUSTRIAL WASTEWATERS CATALYZED BY POLYPHENOL OXIDASE [J].

ATLOW, SC ;

BONADONNAAPARO, L ;

KLIBANOV, AM .

BIOTECHNOLOGY AND BIOENGINEERING, 1984, 26 (06) :599-603

[3] OXIDATION OF PHENOLS BY CHLOROPEROXIDASE [J].

CARMICHAEL, R ;

FEDORAK, PM ;

PICKARD, MA .

BIOTECHNOLOGY LETTERS, 1985, 7 (04) :289-294

[4] ESTIMATING SOIL SEDIMENT PARTITION-COEFFICIENTS FOR ORGANIC-COMPOUNDS BY HIGH-PERFORMANCE REVERSE PHASE LIQUID-CHROMATOGRAPHY [J].

CHIN, YP ;

PEVEN, CS ;

WEBER, WJ .

WATER RESEARCH, 1988, 22 (07) :873-881

[5] Horseradish peroxidase catalyzed hydroxylation of phenol .2. Kinetic model [J].

Courteix, A ;

Bergel, A .

ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (12) :1094-1100

[6] Horseradish peroxidase-catalyzed hydroxylation of phenol .1. Thermodynamic analysis [J].

Courteix, A ;

Bergel, A .

ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (12) :1087-1093

[7]

CRECCHIO C, 1995, BIOTECHNOL BIOENG, V48, P581

[8] OXIDATION OF PHENOL AND ITS REACTION-PRODUCT BY HORSERADISH-PEROXIDASE AND HYDROGEN-PEROXIDE [J].

DANNER, DJ ;

BRIGNAC, PJ ;

ARCENEAU.D ;

PATEL, V .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 156 (02) :759-763

[9]

HAGER LP, 1966, J BIOL CHEM, V241, P1769

[10]

KIRBY P, 2000, ISSO 14001 APPROACH

← 1 2 3 →