Electric field pulses can induce apoptosis

被引：78

作者：

Hofmann, F

Ohnimus, H

Scheller, C

Strupp, W

Zimmermann, U ^{[1
]}

Jassoy, C

机构：

[1] Univ Wurzburg, Lehrstuhl Biotechnol, Biozentrum, D-97074 Wurzburg, Germany

[2] Univ Wurzburg, Inst Virol & Immunbiol, D-97074 Wurzburg, Germany

来源：

JOURNAL OF MEMBRANE BIOLOGY | 1999年 / 169卷 / 02期

关键词：

electropermeabilization; electroporation; apoptosis; jurkat T-lymphoblast; HL-60; caspase;

D O I：

10.1007/s002329900522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Injection of electric field pulses of high in tensity (kV/cm) and short duration (microsecond range) into a cell suspension results in a temporary increase of the membrane permeability due to a reversible electric breakdown of the cell membrane. Here we demonstrate that application of supercritical field pulses between 4.5 and 8.1 kV/cm strength and 40 mu sec duration induce typical features of apoptosis in Jurkat T-lymphoblasts and in HL-60 cells including DNA fragmentation and cleavage of the poly(ADP ribose) polymerase. Apoptosis induction did not depend on the presence of any particular electrolyte in the extracellular medium. However, no apoptosis was observed in solutions without a minimum amount of salt. Apoptotic DNA fragmentation was prevented by the caspase inhibitor zVAD.

引用

页码：103 / 109

页数：7

共 32 条

[1] THE DETERGENT TRITON X-100 INDUCES A DEATH PATTERN IN HUMAN CARCINOMA CELL-LINES THAT RESEMBLES CYTOTOXIC LYMPHOCYTE-INDUCED APOPTOSIS [J].