Extensive hematopoietic stem cell generation in the AGM region via maturation of VE-cadherin+CD45+ pre-definitive HSCs

被引：230

作者：

Taoudi, Samir ^{[1
]}

Gonneau, Christele ^{[1
]}

Moore, Kate ^{[1
]}

Sheridan, Julie M. ^{[1
]}

Blackburn, C. Clare ^{[1
]}

Taylor, Erin ^{[1
]}

Medvinsky, Alexander ^{[1
]}

机构：

[1] Univ Edinburgh, MRC, Ctr Regenerat Med, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland

来源：

CELL STEM CELL | 2008年 / 3卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 英国医学研究理事会;

关键词：

D O I：

10.1016/j.stem.2008.06.004

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Elucidating the mechanisms underlying hematopoietic stem cell (HSC) specification and expansion in the embryo has been hampered by the lack of analytical cell culture systems that recapitulate in vivo development. Here, we describe an ex vivo model that facilitates a rapid and robust emergence of multipotent long-term repopulating HSCs in the embryonic AGM region. Because this method includes a cell dissociation step prior to reconstruction of a three-dimensional functional tissue and preserves both stromal and hematopoietic elements, it allowed us to identify the direct ancestry of the rapidly expanding HSC pool. We demonstrate that extensive generation of definitive HSCs in the AGM occurs predominantly through the acquisition of stem characteristics by the VE-cadherin(+)CD45(+) population.

引用

页码：99 / 108

页数：10

共 31 条

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