Whole genome fingerprinting and genotyping of multiple drug resistant (MDR) isolates of Pseudomonas aeruginosa from endophthalmitis patients in India

被引：12

作者：

Ahmed, Niyaz ^{[1
]}

Bal, Abhijit ^{[2
]}

Khan, Aleem A. ^{[3
,4
]}

Alam, Mahfooz ^{[1
]}

Kagal, Anju ^{[2
]}

Arjunwadkar, Vidya ^{[2
]}

Rajput, Amarsingh ^{[2
]}

Majeed, Ahmed A. ^{[1
]}

Rahman, Syed Asad ^{[1
]}

Banerjee, Sharmistha ^{[1
]}

Joshi, Suvarna ^{[2
]}

Bharadwaj, Renu ^{[2
]}

机构：

[1] Ctr DNA Fingerprinting & Diagnost, Hyderabad 500076, Andhra Pradesh, India

[2] BJ Med Coll, Dept Microbiol, Pune 411001, Maharashtra, India

[3] Deccan Coll Med Sci, Hyderabad 500058, Andhra Pradesh, India

[4] Allied Hosp, Hyderabad 500058, Andhra Pradesh, India

来源：

INFECTION GENETICS AND EVOLUTION | 2002年 / 1卷 / 03期

关键词：

P; aeruginosa; Endophthalmitis; Multiple drug resistance; FAFLP; Polymorphism; Genotyping; Electronic database;

D O I：

10.1016/S1567-1348(02)00030-8

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Genome sequence-based fluorescent amplified-fragment length polymorphism (FAFLP) analysis was investigated for fingerprinting and subtyping 42 multiple drug resistant (MDR) isolates of Pseudomonas aeruginosa from post-surgical endophthalmitis. The FAFLP profiles derived from EcoRI/MseI restricted fragments differentiated clinical isolates and were found to be extremely reproducible. Seventeen different amplification patterns (amplitypes) were observed for all the 42 isolates from 11 different patients. Also, we were able to genotype the isolates based on the differential amplification of a total of 31 FAFLP markers representing genomic fragments from the P. aeruginosa chromosome. This data appears to provide clues to the genetic diversity of endopthalmitis associated strains and could be converted into digitised fingerprints suitable for electronic manipulations and archiving. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：237 / 242

页数：6

共 14 条

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