Rapid diagnosis of toxinogenic Clostridium difficile in faecal samples with internally controlled real-time PCR

被引：53

作者：

van den Berg, RJ ^{[1
]}

Kuijper, EJ ^{[1
]}

van Coppenraet, LESB ^{[1
]}

Claas, ECJ ^{[1
]}

机构：

[1] Leiden Univ, Ctr Med, Ctr Infect Dis, Dept Med Microbiol, NL-2300 RC Leiden, Netherlands

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2006年 / 12卷 / 02期

关键词：

assay; Clostridium difficile; faecal samples; real-time PCR; tcdB gene;

D O I：

10.1111/j.1469-0691.2005.01301.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A real-time PCR assay for Clostridium difficile was developed, based on the tcdB gene, which detected all known toxinogenic reference strains (n = 45), within 30 serogroups and 24 toxinotypes. The analytical sensitivity was 1 x 10(3) CFU/mL, and the detection limit in faeces was 1 x 10(5) CFU/g. The optimal protocol for DNA extraction from faecal samples involved use of the MagnaPure system with a Stool Transport and Recovery (STAR) buffer pre-treatment. In a 1-month prospective study of 85 patients with diarrhoea, the sensitivity, specificity and positive and negative predictive values of the assay were 100%, 94%, 55% and 100%, respectively, compared with the standard cell cytotoxicity assay.

引用

页码：184 / 186

页数：4

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