Fluorescence resonance energy transfer from allophycocyanin to malachite green

被引：20

作者：

Rolinski, OJ

Birch, DJS

McCartney, LJ

Pickup, JC

机构：

[1] Univ Strathclyde, Dept Phys & Appl Phys, Photophys Res Grp, Glasgow G4 0NG, Lanark, Scotland

[2] Guys Hosp, Guys Kings & St Thomas Sch Med, Dept Chem Pathol, London SE1 9RT, England

来源：

CHEMICAL PHYSICS LETTERS | 1999年 / 309卷 / 5-6期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1016/S0009-2614(99)00707-1

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The near-infrared fluorescence resonance energy transfer kinetics of the phycobiliprotein allophycocyanin (APC) to malachite green (MG) have been investigated. A model is proposed to account for the fluorescence decay whereby MG binds to APC with a donor-acceptor site distribution which can be best described by 2D quenching kinetics. The results highlight a potential fallacy when interpreting the dimensionality of complex systems or the location of binding sites from Forster decay kinetics. The use of APC in trans-dermal measurements is proposed. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：395 / 401

页数：7

共 15 条

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[2]

Birch D. J. S., 1991, TOPICS FLUORESCENCE, V1

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