Comparative hybridization of an array of 21 500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas

被引：422

作者：

Schummer, M

Ng, VLV

Baumgarner, RE

Nelson, PS

Schummer, B

Bednarski, DW

Hassell, L

Baldwin, RL

Karlan, BY

Hood, L

机构：

[1] Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA

[2] Univ Heidelberg, Fak Klin Med, Inst Pharmakol & Toxikol, D-68169 Mannheim, Germany

[3] Univ Calif Los Angeles, Cedars Sinai Med Ctr, Dept Obstet & Gynecol, Sch Med, Los Angeles, CA 90048 USA

来源：

GENE | 1999年 / 238卷 / 02期

关键词：

cancer maker; DNA array; differential expression; HE4;

D O I：

10.1016/S0378-1119(99)00342-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Comparative hybridization of cDNA arrays is a powerful tool for the measurement of differences in gene expression between two or more tissues. We optimized this technique and employed it to discover genes with potential for the diagnosis of ovarian cancer. This cancer is rarely identified in time for a good prognosis after diagnosis. An array of 21 500 unknown ovarian cDNAs was hybridized with labeled first-strand cDNA from 10 ovarian tumors and six normal tissues. One hundred and thirty-four clones are overexpressed in at least five of the 10 tumors. These cDNAs were sequenced and compared to public sequence databases. One of these, the gene HE4, was found to be expressed primarily in some ovarian cancers, and is thus a potential marker of ovarian carcinoma. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：375 / 385

页数：11

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