Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells

被引:157
作者
Hodge, CA [1 ]
Colot, HV [1 ]
Stafford, P [1 ]
Cole, CN [1 ]
机构
[1] Dartmouth Med Sch, Dept Biochem, Hanover, NH 03755 USA
关键词
DEAD-box protein; mRNA export; nucleocytoplasmic shuttling; RAT8; YMR255w-GFD1;
D O I
10.1093/emboj/18.20.5778
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In a screen for temperature-sensitive mutants of Saccharomyces cerevisiae defective for mRNA export, we previously identified the essential DEAD-box protein Dbp5p/Rat8p and the nucleoporin Rat7p/Nup159p. Both are essential for mRNA export. Here we report that Dbp5p and Rat7p interact through their N-terminal domains. Deletion of this portion of Rat7p (Rat7p Delta N) results in strong defects in mRNA export and eliminates association of Dbp5p with nuclear pores, Overexpression of Dbp5p completely suppressed the growth and mRNA export defects of rat7 Delta N cells and resulted in weaker suppression in cells carrying rat7-1 or the rss1-37 allele of GLE1, Dbp5p interacts with Gle1p independently of the N-terminus of Dbp5p, Dbp5p shuttles between nucleus and cytoplasm in an Xpo1p-dependent manner. It accumulates in nuclei of xpo1-1 cells and in cells with mutations affecting Mex67p (mex67-5), Gsp1p (Ran) or Ran effectors. Overexpression of Dbp5p prevents nuclear accumulation of mRNA in xpo1-1 cells, but does not restore growth, suggesting that the RNA export defect of xpo1-1 cells may be indirect, In a screen for high-copy suppressors of the rat8-2 allele of DBP5, we identified YMR255w, now called GFD1, Gfd1p is not essential, interacts with Gle1p and Rip1p/Nup42p, and is found in the cytoplasm and at the nuclear rim.
引用
收藏
页码:5778 / 5788
页数:11
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