Potato cysteine proteinase inhibitor gene family: Molecular cloning, characterisation and immunocytochemical localisation studies

被引：51

作者：

Gruden, K

Strukelj, B

Ravnikar, M

PoljsakPrijatelj, M

Mavric, I

Brzin, J

Pungercar, J

Kregar, I

机构：

[1] UNIV LJUBLJANA,DEPT BIOL,BIOTECH FAC,SI-1000 LJUBLJANA,SLOVENIA

[2] NATL INST BIOL,SI-1000 LJUBLJANA,SLOVENIA

[3] UNIV LJUBLJANA,FAC MED,INST MICROBIOL,SI-1000 LJUBLJANA,SLOVENIA

来源：

PLANT MOLECULAR BIOLOGY | 1997年 / 34卷 / 02期

关键词：

cysteine proteinase inhibitor; immunocytochemical localisation; jasmonic acid; potato; soybean Kunitz-type inhibitor;

D O I：

10.1023/A:1005853026333

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Potato cysteine proteinase inhibitors (PCPIs) represent a distinct group of proteins as they show no homology to any other known cysteine proteinase inhibitor superfamilies, but they all belong to the Kunitz-type soybean trypsin inhibitor family. cDNA clones for five PCPIs have been isolated and sequenced. Amino acid substitutions occurring in the Limited regions forming loops on the surface of these proteins suggest a further classification of PCPIs into three subgroups. Accumulation of PCPI was observed in vacuoles of stems after treatment with jasmonic acid (JA) using immunocytochemical localisation, implying that these inhibitors are part of a potato defence mechanism against insects and pathogens. Genomic DNA analysis show that PCPIs form a multigene family and suggest that their genes do not possess any introns.

引用

页码：317 / 323

页数：7

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