A DNA-binding protein from Candida albicans that binds to the RPG box of Saccharomyces cerevisiae and the telomeric repeat sequence of C-albicans

被引:14
作者
Ishii, N
Yamamoto, M
Lahm, HW
Iizumi, S
Yoshihara, F
Nakayama, H
Arisawa, M
Aoki, Y
机构
[1] NIPPON ROCHE RES CTR,DEPT MYCOL,KAMAKURA,KANAGAWA 247,JAPAN
[2] HOFFMANN LA ROCHE AG,DEPT PRPB,CH-4002 BASEL,SWITZERLAND
来源
MICROBIOLOGY-UK | 1997年 / 143卷
关键词
Candida albicans; transcription factor; RPG box; nuclear protein;
D O I
10.1099/00221287-143-2-417
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Electromobility shift assays with a DNA probe containing the Saccharomyces cerevisiae ENO1 RPG box identified a specific DNA-binding protein in total protein extracts of Candida albicans. The protein, named Rbf1p (RPG-box-binding protein 1), bound to other S. cerevisiae RPG boxes, although the nucleotide recognition profile was not completely the same as that of S. cerevisiae Rap1p (repressor-activator protein 1), an RPG-box-binding protein. The repetitive sequence of the C. albicans chromosomal telomere also competed with RPG-box binding to Rbf1p. For further analysis, we purified Rbf1p 57 600-fold from C. albicans total protein extracts, raised mAbs against the purified protein and immunologically cloned the gene, whose ORF specified a protein of 527 aa. The bacterially expressed protein showed RPG-box-binding activity with the same profile as that of the purified one. The Rbf1p, containing two glutamine-rich regions that are found in many transcription factors, showed transcriptional activation capability in S. cerevisiae and was predominantly observed in nuclei. These results suggest that Rbf1p is a transcription factor with telomere-binding activity in C. albicans.
引用
收藏
页码:417 / 427
页数:11
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