Effects of extracellular calcium on viability and osteogenic differentiation of bone marrow stromal cells in vitro

被引：29

作者：

Cheng, Shaowen ^{[1
]}

Wang, Wei ^{[2
]}

Lin, Zhongqin ^{[3
]}

Zhou, Ping ^{[4
]}

Zhang, Xiaolei ^{[2
]}

Zhang, Wei ^{[2
]}

Chen, Qingyu ^{[2
]}

Kou, Dongquan ^{[2
]}

Ying, Xiaozhou ^{[2
]}

Shen, Yue ^{[2
]}

Cheng, Xiaojie ^{[2
]}

Yu, Ziming ^{[1
]}

Peng, Lei ^{[1
]}

Lu, Chuanzhu ^{[1
]}

机构：

[1] Hainan Med Coll, Affiliated Hosp, Ctr Trauma, Haikou 571100, Peoples R China

[2] Wenzhou Med Coll, Affiliated Hosp 2, Dept Orthopaed Surg, Wenzhou 325000, Peoples R China

[3] Hosp Integrated Tradit Chinese & Western Med Wenz, Dept Orthopaed Surg, Wenzhou, Peoples R China

[4] Zhejiang Univ, Affiliated Hosp 1, Dept Oncol, Hangzhou 310003, Zhejiang, Peoples R China

来源：

HUMAN CELL | 2013年 / 26卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Mesenchymal stem cell; Osteogenesis; Calcium; Viability; Differentiation; MESENCHYMAL STEM-CELLS; OSTEOBLAST PROLIFERATION; GENE-EXPRESSION; DEFECTS; MATRIX; REPAIR; HYDROXYAPATITE; OSTEOPONTIN; OSTEOCALCIN; PHOSPHATE;

D O I：

10.1007/s13577-012-0041-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Bone marrow stromal cells (BMSCs) have been extensively used for tissue engineering. However, the effect of Ca2+ on the viability and osteogenic differentiation of BMSCs has yet to be evaluated. To determine the dose-dependent effect of Ca2+ on viability and osteogenesis of BMSCs in vitro, BMSCs were cultured in calcium-free DMEM medium supplemented with various concentrations of Ca2+ (0, 1, 2, 3, 4, and 5 mM) from calcium citrate. Cell viability was analyzed by MTT assay and osteogenic differentiation was evaluated by alkaline phosphatase (ALP) assay, Von Kossa staining, and real-time PCR. Ca2+ stimulated BMSCs viability in a dose-dependent manner. At slightly higher concentrations (4 and 5 mM) in the culture, Ca2+ significantly inhibited the activity of ALP on days 7 and 14 (P < 0.01 or P < 0.05), significantly suppressed collagen synthesis (P < 0.01 or P < 0.05), and significantly elevated calcium deposition (P < 0.01) and mRNA levels of osteocalcin (P < 0.01 or P < 0.05) and osteopontin (P < 0.01 or P < 0.05). Therefore, elevated concentrations of extracellular calcium may promote cell viability and late-stage osteogenic differentiation, but may suppress early-stage osteogenic differentiation in BMSCs.

引用

页码：114 / 120

页数：7

共 32 条

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Osteopontin deficiency increases mineral content and mineral crystallinity in mouse bone [J].