Protective effect of hydroxytyrosol and its metabolite homovanillic alcohol on H2O2 induced lipid peroxidation in renal tubular epithelial cells

被引：51

作者：

Deiana, Monica ^{[1
]}

Incani, Alessandra ^{[1
]}

Rosa, Antonella ^{[1
]}

Corona, Giulia ^{[1
]}

Atzeri, Angela ^{[1
]}

Loru, Debora ^{[1
]}

Melis, M. Paola ^{[1
]}

Dessi, M. Assunta ^{[1
]}

机构：

[1] Univ Cagliari, Sez Patol Sperimentale, Dipartimento Biol Sperimentale, I-09042 Cagliari, Italy

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2008年 / 46卷 / 09期

关键词：

hydroxytyrosol; homovanillic alcohol; lipid peroxidation; unsaturated fatty acids; cholesterol; LLC-PK1;

D O I：

10.1016/j.fct.2008.05.037

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

We investigated the capacity of hydroxytyrosol (HT), 3,4-dihydroxyphenylethanol, and homovanillic alcohol (HVA), 4-hydroxy-3-methoxy-phenylethanol, to inhibit H2O2 induced oxidative damage in LLC-PK1, a porcine kidney epithelial cell line, studying the effect of H2O2 Oil specific cell membrane lipid targets, unsaturated fatty acids and cholesterol. Exposure to H2O2 induced a significant increase of the level of MDA together with a disruption of the membrane structure, with the loss of unsaturated fatty acids, cholesterol and of alpha-tocopherol, and the formation of fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with HT protected renal cells from oxidative damage: the level of membrane lipids was preserved and there was no significant detection of oxidation products. HVA exerted a comparable activity, thus both HT and HVA were able to prevent in renal cells the lipid peroxidation process that plays a central role in tubular cell injury. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2984 / 2990

页数：7

共 55 条

[1] Reduction of cisplatin-induced nephrotoxicity by ginsenosides isolated from processed ginseng in cultured renal tubular cells [J].