A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus

被引：865

作者：

Mougous, Joseph D.

Cuff, Marianne E.

Raunser, Stefan

Shen, Aimee

Zhou, Min

Gifford, Casey A.

Goodman, Andrew L.

Joachimiak, Grazyna

Ordonez, Claudia L.

Lory, Stephen

Walz, Thomas

Joachimiak, Andrzej ^{[1
]}

Mekalanos, John J.

机构：

[1] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

[3] Argonne Natl Lab, Midw Ctr Struct Genom & Struct Biol Ctr, Biosci Div, Argonne, IL 60439 USA

[4] Harvard Univ, Sch Med, Childrens Hosp Boston, Div Resp Dis, Boston, MA 02115 USA

[5] Harvard Univ, Sch Med, Childrens Hosp Boston, Cyst Fibrosis Ctr, Boston, MA 02115 USA

[6] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA

来源：

SCIENCE | 2006年 / 312卷 / 5779期

关键词：

D O I：

10.1126/science.1128393

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bacterial pathogens frequently use protein secretion to mediate interactions with their hosts. Here we found that a virulence locus (HSI-I) of Pseudomonas aeruginosa encodes a protein secretion apparatus. The apparatus assembled in discrete subcellular locations and exported Hcp1, a hexameric protein that forms rings with a 40 angstrom internal diameter. Regulatory patterns of HSI-I suggested that the apparatus functions during chronic infections. We detected Hcp1 in pulmonary secretions of cystic fibrosis (CF) patients and Hcp1-specific antibodies in their sera. Thus, HSI-I likely contributes to the pathogenesis of P. aeruginosa in CF patients. HSI-I-related loci are widely distributed among bacterial pathogens and may play a general role in mediating host interactions.

引用

页码：1526 / 1530

页数：5

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