The structure and function of a soybean beta-glucan-elicitor-binding protein

被引：178

作者：

Umemoto, N ^{[1
]}

Kakitani, M ^{[1
]}

Iwamatsu, A ^{[1
]}

Yoshikawa, M ^{[1
]}

Yamaoka, N ^{[1
]}

Ishida, I ^{[1
]}

机构：

[1] HOKKAIDO UNIV,GRAD SCH SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1997年 / 94卷 / 03期

关键词：

D O I：

10.1073/pnas.94.3.1029

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

beta-Glucan elicitor (GE), released from the cell wall of the phytopathogenic fungus Phytophthora megasperma by soybean glucanases, causes defense reactions in soybean. A GE-binding protein (GEBP) was purified from the membrane fraction of soybean root cells, and its cDNA was isolated. Expression of the cDNA clone in tobacco suspension cultured cells and in Escherichia coli conferred GE-binding activity to both. An antibody against the recombinant protein was found to inhibit the GE binding with the soybean cotyledon membrane fraction as well as the resulting accumulation of phytoalexin. Immunolocalization assays indicated that the GEBPs are located in the plasma membrane of root cells. These results suggest that the cDNA encodes a GE receptor and may mediate the signaling of the elicitor.

引用

页码：1029 / 1034

页数：6

共 42 条

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