Isolation of reductase for SoxR that governs an oxidative response regulon from Escherichia coli

被引：37

作者：

Kobayashi, K ^{[1
]}

Tagawa, S ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan

来源：

FEBS LETTERS | 1999年 / 451卷 / 03期

关键词：

SoxR transcriptional activator; oxidative stress; redox regulation; NADPH; Escherichia coli;

D O I：

10.1016/S0014-5793(99)00565-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SoxR is a transcription factor triggered by oxidative stress in Escherichia coli, Recent evidence suggests that novel redox regulation couples oxidation state to promoter activation. We have isolated the reductase for SoxR in E. coli using an assay of NADPH- and SoxR-dependent cytochrome c reductase activity. When the purified protein was incubated in an anaerobic reaction mixture containing SoxR and NADPH, the reduction of [2Fe-2S] cluster of SoxR was observed by optical and EPR spectroscopy. Our results indicate that the purified protein serves as an NADPH-dependent reduction system for SoxR. (C) 1999 Federation of European Biochemical Societies.

引用

页码：227 / 230

页数：4

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