Macrolide Antibiotic-Mediated Downregulation of MexAB-OprM Efflux Pump Expression in Pseudomonas aeruginosa

被引：18

作者：

Sugimura, Makoto ^{[2
]}

Maseda, Hideaki ^{[3
]}

Hanaki, Hideaki ^{[1
]}

Nakae, Taiji ^{[1
]}

机构：

[1] Kitasato Inst Univ, Lab Antiinfect Drugs, Sagamihara, Kanagawa 2288555, Japan

[2] Kitasato Inst Univ, Grad Sch Med Sci, Sagamihara, Kanagawa 2288555, Japan

[3] Fac Engn, Dept Biol Sci & Technol, Tokushima 7708605, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2008年 / 52卷 / 11期

关键词：

D O I：

10.1128/AAC.00511-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Macrolide antibiotics modulate the quorum-sensing system of Pseudomonas aeruginosa. We tested the effect of macrolide antibiotics on the cell density-dependent expression of the MexAB-OprM efflux pump and found that 1.0 mu g/ml (MIC/6.25) of azithromycin suppressed the expression of MexAB-OprM by about 70%, with the result that the cells became two- to fourfold more susceptible to antibiotics such as aztreonam, tetracycline, carbenicillin, chloramphenicol, and novobiocin.

引用

页码：4141 / 4144

页数：4

共 32 条

[1] Crystal structure of the drug discharge outer membrane protein, OprM, of Pseudomonas aeruginosa -: Dual modes of membrane anchoring and occluded cavity end [J].

Akama, H ;

Kanemaki, M ;

Yoshimura, M ;

Tsukihara, T ;

Kashiwagi, T ;

Yoneyama, H ;

Narita, S ;

Nakagawa, A ;

Nakae, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (51) :52816-52819

[2] Crystal structure of the membrane fusion protein, MexA, of the multidrug transporter in Pseudomonas aeruginosa [J].

Akama, H ;

Matsuura, T ;

Kashiwagi, S ;

Yoneyama, H ;

Narita, SI ;

Tsukihara, T ;

Nakagawa, A ;

Nakae, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (25) :25939-25942

[3]

[Anonymous], JPN J THORAC DIS

[4] A dose-response study of antibiotic resistance in Pseudomonas aeruginosa biofilms [J].

Brooun, A ;

Liu, SH ;

Lewis, K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (03) :640-646

[5] Interference with Pseudomonas quinolone signal synthesis inhibits virulence factor expression by Pseudomonas aeruginosa [J].

Calfee, MW ;

Coleman, JP ;

Pesci, EC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (20) :11633-11637

[6] Protein modulator of multidrug efflux gene expression in Pseudomonas aeruginosa [J].

Daigle, Denis M. ;

Cao, Lily ;

Fraud, Sebastien ;

Wilke, Mark S. ;

Pacey, Angela ;

Klinoski, Rachael ;

Strynadka, Natalie C. ;

Dean, Charles R. ;

Poole, Keith .

JOURNAL OF BACTERIOLOGY, 2007, 189 (15) :5441-5451

[7] A FAMILY OF EXTRACYTOPLASMIC PROTEINS THAT ALLOW TRANSPORT OF LARGE MOLECULES ACROSS THE OUTER MEMBRANES OF GRAM-NEGATIVE BACTERIA [J].

DINH, T ;

PAULSEN, IT ;

SAIER, MH .

JOURNAL OF BACTERIOLOGY, 1994, 176 (13) :3825-3831

[8] An elegant means of self-protection in gram-negative bacteria by recognizing and extruding xenobiotics from the periplasmic space [J].

Eda, S ;

Maseda, H ;

Nakae, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (04) :2085-2088

[9] Long term azithromycin in children with cystic fibrosis: a randomised, placebo-controlled crossover trial [J].

Equi, A ;

Balfour-Lynn, IM ;

Bush, A ;

Rosenthal, M .

LANCET, 2002, 360 (9338) :978-984

[10] Biofilm formation by Pseudomonas aeruginosa:: role of the C4-HSL cell-to-cell signal and inhibition by azithromycin [J].

Favre-Bonté, S ;

Köhler, T ;

Van Delden, C .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2003, 52 (04) :598-604

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