Renal cell carcinoma and normal kidney protein expression

被引:117
作者
Sarto, C
Marocchi, A
Sanchez, JC
Giannone, D
Frutiger, S
Golaz, O
Wilkins, MR
Doro, G
Cappellano, F
Hughes, G
Hochstrasser, DF
Mocarelli, P
机构
[1] OSPED NIGUARDA CA GRANDA,CLIN CHEM & HEMATOL LAB,MILAN,ITALY
[2] UNIV HOSP GENEVA,CLIN CHEM LAB,GENEVA,SWITZERLAND
[3] UNIV GENEVA,DEPT BIOCHEM MED,GENEVA,SWITZERLAND
[4] DESIO HOSP,DEPT UROL,DESIO,ITALY
关键词
kidney; two-dimensional polyacrylamide gel electrophoresis; renal cell carcinoma; database;
D O I
10.1002/elps.1150180343
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Renal cell carcinoma (RCC), a human kidney cancer from the proximal tubular epithelium, accounts for about 3% of adult malignancies. Molecular and cytogenetic analysis have highlighted deletions, translocations, or loss of heterozygosity in the 3p21-p26, a putative RCC locus, as well as in 6q, 8p, 9pq, and 14pq. Studies on phenotypic expression of human kidney tissue and on post-translational modifications in RCC have not yet provided a marker for early renal cell carcinoma diagnosis. Current dignostic methods do not help to detect the tumor before advanced stages. We therefore used two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) to study normal and tumor kidney tissues in ten patients suffering from RCC. A human kidney protein map in the SWISS-2DPAGE database accessible through the ExPASy WWW Molecular Biology Server was established. Of 2789 separated polypeptides, 43 were identified by gel comparison, amino acid analysis, N-terminal sequencing, and/or immunodetection. The comparison between normal and tumor kidney tissues showed four polypeptides to be absent in RCC. One of them was identified as ubiquinol cytochrome c reductase (UQCR), whose locus has elsewhere been tentatively assigned to chromosome 19p12 or chromosome 22. A second polypeptide was identified as mitochondrial NADH-ubiquinone oxidoreductase complex I whose locus is located on chromosome 18p11.2 and chromosome 19q13.3. These result suggest that the lack of UQCR and of mitochondrial NADH-ubiquinone oxidoreductase complex I expression in RCC may be caused by unknown deletions, or by changes in gene transcription or translation. It might indicate that mitochondrial disfunction plays a major role in RCC genesis or evolution.
引用
收藏
页码:599 / 604
页数:6
相关论文
共 43 条
[1]  
AUGLAND P, 1991, CANCER RES, V51, P1071
[2]   MICROPREPARATIVE 2-DIMENSIONAL ELECTROPHORESIS ALLOWING THE SEPARATION OF SAMPLES CONTAINING MILLIGRAM AMOUNTS OF PROTEINS [J].
BJELLQVIST, B ;
SANCHEZ, JC ;
PASQUALI, C ;
RAVIER, F ;
PAQUET, N ;
FRUTIGER, S ;
HUGHES, GJ ;
HOCHSTRASSER, D .
ELECTROPHORESIS, 1993, 14 (12) :1375-1378
[3]   A NONLINEAR WIDE-RANGE IMMOBILIZED PH GRADIENT FOR 2-DIMENSIONAL ELECTROPHORESIS AND ITS DEFINITION IN A RELEVANT PH SCALE [J].
BJELLQVIST, B ;
PASQUALI, C ;
RAVIER, F ;
SANCHEZ, JC ;
HOCHSTRASSER, D .
ELECTROPHORESIS, 1993, 14 (12) :1357-1365
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]  
CELIS JE, 1984, 2 DIMENSIONAL GEL EL, P3
[6]  
DELAHUNT B, 1995, CANCER, V75, P2714, DOI 10.1002/1097-0142(19950601)75:11<2714::AID-CNCR2820751113>3.0.CO
[7]  
2-X
[8]  
DEVINE PL, 1993, CANCER BIOCHEM BIOPH, V13, P221
[9]   ASSIGNMENT OF THE GENE (UQCRFS1) FOR THE RIESKE IRON-SULFUR PROTEIN SUBUNIT OF THE MITOCHONDRIAL CYTOCHROME BC(1) COMPLEX TO THE 22Q13 AND 19Q12-Q13.1 REGIONS OF THE HUMAN GENOME [J].
DUNCAN, AMV ;
ANDERSON, L ;
DUFF, C ;
OZAWA, T ;
SUZUKI, H ;
WORTON, R ;
ROZEN, R .
GENOMICS, 1994, 21 (01) :281-283
[10]   THE BIOCHEMISTRY OF P-GLYCOPROTEIN-MEDIATED MULTIDRUG RESISTANCE [J].
ENDICOTT, JA ;
LING, V .
ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :137-171