Tanshinone IIA inhibits the growth of pancreatic cancer BxPC-3 cells by decreasing protein expression of TCTP, MCL-1 and Bcl-xL

被引:27
作者
Huang, Cheng-Yen [1 ,2 ]
Chiu, Tsung-Lang [3 ,4 ]
Kuo, Shou-Jen [1 ]
Chien, Su-Yu [5 ]
Chen, Dar-Ren [1 ,6 ]
Su, Chin-Cheng [1 ,2 ,6 ,7 ]
机构
[1] Changhua Christian Hosp, Dept Surg, Changhua 50006, Taiwan
[2] Changhua Christian Hosp, Tumor Res Ctr Integrat Med, Changhua 50006, Taiwan
[3] Tzu Chi Univ, Hualien 970, Taiwan
[4] Buddhist Tzu Chi Gen Hosp, Neuromed Sci Ctr, Hualien 970, Taiwan
[5] Changhua Christian Hosp, Dept Pharm, Changhua 50006, Taiwan
[6] Changhua Christian Hosp, Comprehens Breast Canc Ctr, Changhua 50006, Taiwan
[7] China Med Univ, Coll Chinese Med, Sch Chinese Med, Taichung 40402, Taiwan
关键词
tanshinone IIA; BxPC-3; cells; TCTP; MCL-1; MESSENGER-RNA; IN-VITRO; FORTILIN; APOPTOSIS; BAX;
D O I
10.3892/mmr.2013.1290
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
Pancreatic cancer remains a challenging disease worldwide. Tanshinone IIA (Tan-IIA) is one of the active constituents of Danshen (Radix Salviae miltiorrhizae). Tan-IIA has been hypothesized to inhibit numerous human cancer cells by various molecular mechanisms. However, the efficacy and molecular mechanism of Tan-IIA action in pancreatic cancer has not been well studied. In the present study, the cytotoxicity of Tan-IIA in human pancreatic cancer BxPC-3 cells was evaluated by MTT assay. Cell cycle analysis of BxPC-3 cells treated with Tan-IIA was performed by flow cytometry (FACS). Protein expression levels of TCTP, MCL-1, Bcl-xL, Bax and Caspase-3 in BxPC-3 cells were measured by western blot analysis. The results revealed that Tan-IIA inhibited BxPC-3 cells in a time- and dose-dependent manner. FACS analysis demonstrated that Tan-IIA increases the rate of sub-G, phase. BxPC-3 cells treated with Tan-IIA were identified to upregulate protein expression of Bax and Caspase-3 and downregulate expression of TCTP, MCL-1 and Bcl-xL. These results indicate that Tan-IIA may inhibit BxPC-3 human pancreatic cancer cells through the induction of apoptosis by decreasing protein expression of TCTP, MCL-1 and Bcl-xL and increasing Bax expression in vitro. The chemotherapeutic potential of Tan-IIA for human pancreatic cancer warrants further study.
引用
收藏
页码:1045 / 1049
页数:5
相关论文
共 22 条
[1]
BOMMER UA, 1994, CELL MOL BIOL RES, V40, P633
[2]
Separation and determination of active components in Radix Salviae miltiorrhizae and its medicinal preparations by nonaqueous capillary electrophoresis [J].
Chen, AJ ;
Zhang, JY ;
Li, CH ;
Chen, XF ;
Hu, ZD ;
Chen, XG .
JOURNAL OF SEPARATION SCIENCE, 2004, 27 (7-8) :569-575
[3]
Tanshinone IIA inhibits Hep-J5 cells by increasing calreticulin, caspase 12 and GADD153 protein expression [J].
Cheng, Chun-Yuan ;
Su, Chin-Cheng .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2010, 26 (03) :379-385
[4]
Tanshinone IIA may inhibit the growth of small cell lung cancer H146 cells by up-regulating the Bax/Bcl-2 ratio and decreasing mitochondrial membrane potential [J].
Cheng, Chun-Yuan ;
Su, Chin-Cheng .
MOLECULAR MEDICINE REPORTS, 2010, 3 (04) :645-650
[5]
NUCLEOTIDE-SEQUENCE OF A MAJOR MESSENGER-RNA FOR A 21 KILODALTON POLYPEPTIDE THAT IS UNDER TRANSLATIONAL CONTROL IN MOUSE-TUMOR CELLS [J].
CHITPATIMA, ST ;
MAKRIDES, S ;
BANDYOPADHYAY, R ;
BRAWERMAN, G .
NUCLEIC ACIDS RESEARCH, 1988, 16 (05) :2350-2350
[6]
Tanshinone IIA induces apoptosis in human lung cancer A549 cells through the induction of reactive oxygen species and decreasing the mitochondrial membrane potential [J].
Chiu, Tsung-Lang ;
Su, Chin-Cheng .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2010, 25 (02) :231-236
[7]
In vitro cytotoxic activity of abietane diterpenes from Peltodon longipes as well as Salvia miltiorrhiza and Salvia sahendica [J].
Fronza, M. ;
Murillo, R. ;
Slusarczyk, S. ;
Adams, M. ;
Hamburger, M. ;
Heinzmann, B. ;
Laufer, S. ;
Merfort, I. .
BIOORGANIC & MEDICINAL CHEMISTRY, 2011, 19 (16) :4876-4881
[8]
Antiapoptotic protein partners fortilin and MCL1 independently protect cells from 5-fluorouracil-induced cytotoxicity [J].
Graidist, P ;
Phongdara, A ;
Fujise, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (39) :40868-40875
[9]
Characterization of fortilin, a novel antiapoptotic protein [J].
Li, F ;
Zhang, D ;
Fujise, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (50) :47542-47549
[10]
Stabilization and enhancement of the antiapoptotic activity of Mcl-1 by TCTP [J].
Liu, H ;
Peng, HW ;
Cheng, YS ;
Yuan, HS ;
Yang-Yen, HF .
MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (08) :3117-3126